ArticleThe Journal of cell biology1996
The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 222 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
222 citing papers in PubMed, 449 citations in OpenAlex.
- Telomeres in mitosis: organization and dynamics.Nucleus (Austin, Tex.) · 2026Review
- A translocation within the Ogataea species complex alters local subtelomeric chromatin while maintaining overall genome organization.G3 (Bethesda, Md.) · 2026Article
- The nuclear basket nucleoporin MLP1 is required to maintain nuclear integrity, and mitotic fidelity in Trypanosoma brucei.PLoS neglected tropical diseases · 2026Article
- Sir proteins impede, but do not prevent, access to silent chromatin in living Saccharomyces cerevisiae.Scientific reports · 2026Article
- A Translocation within thebioRxiv : the preprint server for biology · 2026Article
- Parasitic plasmids are anchored to inactive regions of eukaryotic chromosomes through a nucleosome signal.The EMBO journal · 2025Article
- DNA methylation-based high-resolution mapping of long-distance chromosomal interactions in nucleosome-depleted regions.Nature communications · 2024Article
- Nuclear transport proteins: structure, function, and disease relevance.Signal transduction and targeted therapy · 2023Review
- The shelterin protein expansion of telomere dynamics: Linking early life adversity, life history, and the hallmarks of aging.Neuroscience and biobehavioral reviews · 2023Review
- SIR telomere silencing depends on nuclear envelope lipids and modulates sensitivity to a lysolipid.The Journal of cell biology · 2023Article
- Senescence-associated transcriptional derepression in subtelomeres is determined in a chromosome-end-specific manner.Aging cell · 2023Article
- Review
- The Nuclear Pore Complex as a Transcription Regulator.Cold Spring Harbor perspectives in biology · 2022Review
- Review
- CRISPR-Cas-Mediated Tethering Recruits the YeastACS synthetic biology · 2021Article
- The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.PLoS genetics · 2021Article
- Nuclear envelope tethering inhibits the formation of ALT-associated PML bodies in ALT cells.Aging · 2021Article
- Sir3 mediates long-range chromosome interactions in budding yeast.Genome research · 2021Article
- High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells.Stem cells international · 2021Article
- Telomere Roles in Fungal Genome Evolution and Adaptation.Frontiers in genetics · 2021Article
162 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a novel technique for combined immunofluorescence/in situ hybridization on fixed budding yeast cells that maintains the three-dimensional structure of the nucleus as monitored by focal sections of cells labeled with fluorescent probes and by staining with a nuclear pore antibody. Within the resolution of these immunodetection techniques, we show that proteins encoded by the SIR3, SIR4, and RAP1 genes colocalize in a statistically significant manner with Y' telomere-associated DNA sequences. In wild-type cells the Y' in situ hybridization signals can be resolved by light microscopy into fewer than ten foci per diploid nucleus. This suggests that telomeres are clustered in vegetatively growing cells, and that proteins essential for telomeric silencing are concentrated at their sites of action, i.e., at telomeres and/or subtelomeric regions. As observed for Rap1, the Sir4p staining is diffuse in a sir3- strain, and similarly, Sir3p staining is no longer punctate in a sir4- strain, although the derivatized Y' probe continues to label discrete sites in these strains. Nonetheless, the Y' FISH is altered in a qualitative manner in sir3 and sir4 mutant strains, consistent with the previously reported phenotypes of shortened telomeric repeats and loss of telomeric silencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.