ArticleJournal of bacteriology1996
Characterization of an OprL null mutant of Pseudomonas putida.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Cellular and molecular outcomes of Pseudomonas putida KT2440 exposure to aluminium.Folia microbiologica · 2025Article
- Perturbation-specific transcriptional mapping for unbiased target elucidation of antibiotics.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A set of activators and repressors control peripheral glucose pathways in Pseudomonas putida to yield a common central intermediate.Journal of bacteriology · 2008Article
- Vesicle-independent extracellular release of a proinflammatory outer membrane lipoprotein in free-soluble form.BMC microbiology · 2008Article
- Convergent peripheral pathways catalyze initial glucose catabolism in Pseudomonas putida: genomic and flux analysis.Journal of bacteriology · 2007Article
- Identification of the initial steps in D-lysine catabolism in Pseudomonas putida.Journal of bacteriology · 2007Article
- The RpoT regulon of Pseudomonas putida DOT-T1E and its role in stress endurance against solvents.Journal of bacteriology · 2007Article
- Role of Pseudomonas putida tol-oprL gene products in uptake of solutes through the cytoplasmic membrane.Journal of bacteriology · 2003Article
- Transcriptional organization of the Pseudomonas putida tol-oprL genes.Journal of bacteriology · 2003Article
- Physiological characterization of Pseudomonas putida DOT-T1E tolerance to p-hydroxybenzoate.Applied and environmental microbiology · 2001Article
- Mutations in each of the tol genes of Pseudomonas putida reveal that they are critical for maintenance of outer membrane stability.Journal of bacteriology · 2000Article
- A set of genes encoding a second toluene efflux system in Pseudomonas putida DOT-T1E is linked to the tod genes for toluene metabolism.Journal of bacteriology · 2000Article
- Involvement of the cis/trans isomerase Cti in solvent resistance of Pseudomonas putida DOT-T1E.Journal of bacteriology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A Pseudomonas putida oprL null mutant was generated with reverse genetics by using an in vitro-truncated oprL::xylE construct and in vivo allelic exchange. The nature of the mutation introduced in P. putida was confirmed by Southern blotting. Western blots (immunoblots) of peptidoglycan-associated proteins revealed that the OprL protein was not made in the mutant strain, whereas it was detectable as a 19-kDa band in protein preparations of the wild-type strain. The P. putida oprL, mutant exhibited altered cell morphology as revealed by electron microscopy and was more sensitive to sodium dodecyl sulfate, deoxycholate, and EDTA than the wild-type strain. The oprL gene was conserved in a wide variety of the Pseudomonas strains belonging to rRNA group I, which suggests that this gene is important for the maintenance of the cell envelope and cell morphology in this group of microorganisms.
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