ArticleJournal of bacteriology1996
Molecular cloning and expression of the spsB gene encoding an essential type I signal peptidase from Staphylococcus aureus.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 110 citations in OpenAlex.
- Dynamic nature of Staphylococcus aureus type I signal peptidases.Biophysical journal · 2025Article
- SpbR controls lipoteichoic acid length by directly inhibiting signal peptidase SpsB inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipoteichoic acid biosynthesis bymBio · 2024Article
- New Perspectives on Escherichia coli Signal Peptidase I Substrate Specificity: Investigating Why the TasA Cleavage Site Is Incompatible with LepB Cleavage.Microbiology spectrum · 2023Article
- Article
- Miniature Seed6, encoding an endoplasmic reticulum signal peptidase, is critical in seed development.Plant physiology · 2021Article
- Staphylococcal Protein Secretion and Envelope Assembly.Microbiology spectrum · 2019Review
- MroQ Is a Novel Abi-Domain Protein That Influences Virulence Gene Expression inInfection and immunity · 2019Article
- Involvement of signal peptidase I in Streptococcus sanguinis biofilm formation.Microbiology (Reading, England) · 2017Article
- Staphylococcus aureus Exploits a Non-ribosomal Cyclic Dipeptide to Modulate Survival within Epithelial Cells and Phagocytes.PLoS pathogens · 2016Article
- Expression, purification and crystallization of a membrane-associated, catalytically active type I signal peptidase from Staphylococcus aureus.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Sec-secretion and sortase-mediated anchoring of proteins in Gram-positive bacteria.Biochimica et biophysica acta · 2014Review
- Structure and activity of Streptococcus pyogenes SipA: a signal peptidase-like protein essential for pilus polymerisation.PloS one · 2014Article
- Efforts toward broadening the spectrum of arylomycin antibiotic activity.Bioorganic & medicinal chemistry letters · 2013Article
- Random mutagenesis and topology analysis of the autoinducing peptide biosynthesis proteins in Staphylococcus aureus.Molecular microbiology · 2013Article
- Requirement of signal peptidase ComC and thiol-disulfide oxidoreductase DsbA for optimal cell surface display of pseudopilin ComGC in Staphylococcus aureus.Applied and environmental microbiology · 2012Article
- Broadening the spectrum of β-lactam antibiotics through inhibition of signal peptidase type I.Antimicrobial agents and chemotherapy · 2012Article
- Pseudomonas aeruginosa possesses two putative type I signal peptidases, LepB and PA1303, each with distinct roles in physiology and virulence.Journal of bacteriology · 2012Article
- Inhibition of the sole type I signal peptidase of Mycobacterium tuberculosis is bactericidal under replicating and nonreplicating conditions.Journal of bacteriology · 2012Article
- Signal peptidase I: cleaving the way to mature proteins.Protein science : a publication of the Protein Society · 2012Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene, spsB, encoding a type I signal peptidase has been cloned from the gram-positive eubacterium Staphylococcus aureus. The gene encodes a protein of 191 amino acid residues with a calculated molecular mass of 21,692 Da. Comparison of the protein sequence with those of known type I signal peptidases indicates conservation of amino acid residues known to be important or essential for catalytic activity. The enzyme has been expressed to high levels in Escherichia coli and has been demonstrated to possess enzymatic activity against E. coli preproteins in vivo. Experiments whereby the spsB gene was transferred to a plasmid that is temperature sensitive for replication indicate that spsB is an essential gene. We identified an open reading frame immediately upstream of the spsB gene which encodes a type I signal peptidase homolog of 174 amino acid residues with a calculated molecular mass of 20,146 Da that is predicted to be devoid of catalytic activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.