ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1996
Conditional ablation of cerebellar astrocytes in postnatal transgenic mice.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 122 citations in OpenAlex.
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- Spatiotemporally selective astrocytic ATP dynamics encode injury information sensed by microglia following brain injury in mice.Nature neuroscience · 2024Article
- Astrocyte growth is driven by the Tre1/S1pr1 phospholipid-binding G protein-coupled receptor.Neuron · 2024Article
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- The Implication of Glial Metabotropic Glutamate Receptors in Alzheimer's Disease.Current neuropharmacology · 2023Review
- Intranasal Leukemia Inhibitory Factor Attenuates Gliosis and Axonal Injury and Improves Sensorimotor Function After a Mild Pediatric Traumatic Brain Injury.Neurotrauma reports · 2023Article
- The gut-immune-brain axis in neurodevelopment and neurological disorders.Microbiome research reports · 2022Review
- Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues.Nature communications · 2020Article
- Cerebellar Astrocytes: Much More Than Passive Bystanders In Ataxia Pathophysiology.Journal of clinical medicine · 2020Review
- Astrocytes Are Required for Oligodendrocyte Survival and Maintenance of Myelin Compaction and Integrity.Frontiers in cellular neuroscience · 2020Article
- Glial Cell-Axonal Growth Cone Interactions in Neurodevelopment and Regeneration.Frontiers in neuroscience · 2020Review
- The Role of Astrocytes in the Development of the Cerebellum.Cerebellum (London, England) · 2019Review
- Mutant ataxin1 disrupts cerebellar development in spinocerebellar ataxia type 1.The Journal of clinical investigation · 2018Article
- Prolonged Dysfunction of Astrocytes and Activation of Microglia Accelerate Degeneration of Dopaminergic Neurons in the Rat Substantia Nigra and Block Compensation of Early Motor Dysfunction Induced by 6-OHDA.Molecular neurobiology · 2018Article
- The Molecular Pathway Regulating Bergmann Glia and Folia Generation in the Cerebellum.Cerebellum (London, England) · 2018Review
- Astrocytes Promote Medulloblastoma Progression through Hedgehog Secretion.Cancer research · 2017Article
- Article
- In vivo imaging reveals rapid astrocyte depletion and axon damage in a model of neuromyelitis optica-related pathology.Annals of neurology · 2016Article
- ERBB3-mediated regulation of Bergmann glia proliferation in cerebellar lamination.Development (Cambridge, England) · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Astrocytes have been proposed to have multiple roles in the development and maintenance of the vertebrate CNS. To facilitate documentation of these roles, we designed a transgene to enable their ablation at selectable times. The transgene consists of the coding region for the herpes simplex virus-thymidine kinase (HSV-TK) under the control of the human glial fibrillary acidic protein gene promoter. The HSV-TK is innocuous but converts the antiherpetic agent ganciclovir (GCV) to a toxic product that interferes with DNA replication in proliferating cells. In a developmental study, transgenic mice were treated with GCV during the first postnatal week, with evaluation at P19. Treated mice displayed severe ataxia. Histological examination revealed disrupted astrocyte development, particularly in the cerebellum, with marked secondary effects on other cell types. Cerebellar defects included a loss in the numbers of astrocytes and an overall reduction in cerebellar size and disruption of the normally well defined cellular layers. Radial glia were disordered, Purkinje cells were ectopically distributed and displayed abnormal dendritic trees, and granule cells were markedly depleted. These effects were more severe in animals treated on postnatal day 1 versus treatment at day 5. A major factor causing granule cell death was excitotoxicity attributable to activation of NMDA receptors. These results suggest a critical role for astrocytes in cerebellar development.
Indexed as
Identifiers
8824329PMC6579279W1880770180What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.