ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1996
Aggregation of sodium channels induced by a postnatally upregulated isoform of agrin.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 34 citations in OpenAlex.
- Mechanisms and physiological implications of cooperative gating of clustered ion channels.Physiological reviews · 2022Review
- Local induction of acetylcholine receptor clustering in myotube cultures using microfluidic application of agrin.Biophysical journal · 2006Article
- A selective role for MRF4 in innervated adult skeletal muscle: Na(V) 1.4 Na+ channel expression is reduced in MRF4-null mice.Gene expression · 2005Article
- Interaction of muscle and brain sodium channels with multiple members of the syntrophin family of dystrophin-associated proteins.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Morphogenesis of the node of Ranvier: co-clusters of ankyrin and ankyrin-binding integral proteins define early developmental intermediates.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Neural agrin induces ectopic postsynaptic specializations in innervated muscle fibers.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Clustering of voltage-sensitive sodium channels on axons is independent of direct Schwann cell contact in the dystrophic mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Basic helix-loop-helix factors recruit nuclear factor I to enhance expression of the NaV 1.4 Na+ channel gene.Biochimica et biophysica actaArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Agrin is involved in signaling the formation of high concentrations of acetylcholine receptors (AChRs) at the neuromuscular junction (NMJ). There are multiple isoforms of agrin attributable to alternative splicing, and these isoforms are differentially expressed during development and between tissues. The ability to cluster AChRs varies among the agrin isoforms. Sodium channels (NaChs) are also concentrated at the NMJ. We have tested various agrin isoforms for their ability to induce formation of clusters of NaChs. We grew cocultures of dissociated adult rat muscle fibers with chinese hamster ovary (CHO) cells that had been transfected with different isoforms of agrin. Using immunocytochemical techniques, we determined that after 1 d in culture, CHO cells synthesizing the neuronally expressed isoform with an eight amino acid insert (Agrin8) were able to form NaCh clusters at sites of contact between the CHO cell and muscle cell. Clusters of NaChs could be formed anywhere along a muscle fiber, but more clusters were detected close to the endplate where the endogenous level of NaChs was higher. None of the other neuronal-specific agrin isoforms was able to cluster NaChs. Because Agrin8 is the only agrin isoform that is upregulated at birth when NaChs begin to cluster at the NMJ, we conclude that Agrin8 expression by motor neurons is a signal for NaCh clustering at the NMJ during normal development.
Indexed as
Identifiers
8824318PMC6579246W1861644229What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.