ArticleThe Journal of physiology1996
Changes in IP3 and cytosolic Ca2+ in response to sugars and non-sugar sweeteners in transduction of sweet taste in the rat.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 206 citations in OpenAlex.
- The Antioxidative, Anti-inflammatory and Anti-apoptotic Effects of Tetrapleura Tetraptera (Aidan) Ethanol Leaf Extract in the Brain of Wistar Rats Exposed to Aspartame.Molecular neurobiology · 2025Article
- Effect of taste recall training using 5 sweet substances on sweet taste sensitivities.Chemical senses · 2025Article
- Early-life influences of low-calorie sweetener consumption on sugar taste.Physiology & behavior · 2023Review
- Sweet Taste Is Complex: Signaling Cascades and Circuits Involved in Sweet Sensation.Frontiers in human neuroscience · 2021Review
- An alternative pathway for sweet sensation: possible mechanisms and physiological relevance.Pflugers Archiv : European journal of physiology · 2020Review
- Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel, Switzerland) · 2020Review
- Review
- TRPM4 and TRPM5 are both required for normal signaling in taste receptor cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- The Sweetener-Sensing Mechanisms of the Ghrelin Cell.Nutrients · 2016Article
- A High-Throughput Automated Microfluidic Platform for Calcium Imaging of Taste Sensing.Molecules (Basel, Switzerland) · 2016Article
- Temperature Affects Human Sweet Taste via At Least Two Mechanisms.Chemical senses · 2015Article
- Administration of saccharin to neonatal mice influences body composition of adult males and reduces body weight of females.Endocrinology · 2014Article
- Taste dysfunction in BTBR mice due to a mutation of Itpr3, the inositol triphosphate receptor 3 gene.Physiological genomics · 2013Article
- Gustatory sensory cells express a receptor responsive to protein breakdown products (GPR92).Histochemistry and cell biology · 2013Article
- Pragmatically on the sense of taste - a short treatise based on culinary art.Przeglad gastroenterologiczny · 2013Review
- Macronutrient choice of BTBR.NZW mice congenic for a 21-gene region of chromosome 17.Physiology & behavior · 2012Article
- A conditioned aversion study of sucrose and SC45647 taste in TRPM5 knockout mice.Chemical senses · 2012Article
- Mechanisms of taste bud cell loss after head and neck irradiation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Taste receptor signalling - from tongues to lungs.Acta physiologica (Oxford, England) · 2012Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The transduction pathways of sweet-sensitive cells in rat circumvallate (CV) taste buds were investigated with assays for inositol 1,4,5-trisphosphate (IP3) and with Ca2+ imaging. Stimulation with the non-sugar sweeteners SC-45647 and saccharin rapidly increased the cellular content of IP3 by 400 pmol (mg protein)-1, while sucrose had a much smaller effect on IP3. As shown previously, sucrose, but not saccharin, increased the content of cyclic adenosine monophosphate (cAMP) of this preparation. 2. Stimulation of isolated CV taste buds with SC-45647 increased the cytosolic Ca2+ concentration ([Ca2+]i) by 56.7 +/- 3.2 nM (n = 181). Due to the non-confocality of the measuring system, these concentrations are underestimates. The increase in [Ca2+]i did not require the presence of extracellular Ca2+, suggesting that the Ca2+ release was from intracellular stores. 3. Individual cells responding to the non-sugar sweeteners with Ca2+ release also responded to sucrose and to forskolin with an increase in [Ca2+]i. Such cells did not respond to the bitter tastant denatonium chloride. 4. Responses to sucrose were abolished by lowering the Ca2+ concentration of the stimulus solution, indicating Ca2+ uptake from the extracellular medium. 5. The responses of sweet-sensitive cells to forskolin were also abolished when Ca2+ ions were omitted from the stimulus solution. They were partially inhibited by the presence of Co2+, Ni2+, D600 (methoxyverapamil) and amiloride, indicating multiple pathways of Ca2+ uptake activated by cAMP. 6. In conclusion, a sweet-sensitive cell of the rat responds to sucrose with an increase in cAMP and Ca2+ uptake, but to non-sugar sweeteners with an increase in IP3 and Ca2+ release. The increase in [Ca2+]i, common to both pathways, is presumably required for synaptic exocytosis and for signal termination.
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