ArticleJournal of clinical microbiology1996
Preparation of mycobacterial DNA from blood culture fluids by simple alkali wash and heat lysis method for PCR detection.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- High Incidence of Metastatic Infections in Panton-Valentine Leucocidin-Negative, Community-Acquired Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections.Journal of clinical microbiology · 2013Article
- Instrument-free exothermic heating with phase change temperature control for paper microfluidic devices.Proceedings of SPIE--the International Society for Optical Engineering · 2013Article
- Bacterial identification by 16S rRNA gene PCR-hybridization as a supplement to negative culture results.Journal of clinical microbiology · 2011Article
- Evaluation of the alkaline wash/lysis procedure for the molecular diagnosis of a positive bacterial blood culture in clinical routine practice.Journal of clinical laboratory analysis · 2010Article
- The era of molecular and other non-culture-based methods in diagnosis of sepsis.Clinical microbiology reviews · 2010Review
- Rapid Identification of Mycobacterium tuberculosis and nontuberculous mycobacteria by multiplex, real-time PCR.Journal of clinical microbiology · 2009Article
- Multiplex tandem PCR: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens.Journal of clinical microbiology · 2008Article
- Selective enrichment and detection of mycobacterial DNA in paucibacillary specimens.Journal of microbiological methods · 2006Article
- Bio-banking in microbiology: from sample collection to epidemiology, diagnosis and research.FEMS microbiology reviews · 2005Review
- Efficient method for mycobacterial DNA extraction in blood cultures aids rapid PCR identification of Mycobacterium tuberculosis and Mycobacterium avium.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2004Article
- DNA extraction from archival formalin-fixed, paraffin-embedded tissues: heat-induced retrieval in alkaline solution.Histochemistry and cell biology · 2004Article
- Early detection and identification of commonly encountered Candida species from simulated blood cultures by using a real-time PCR-based assay.The Journal of molecular diagnostics : JMD · 2004Article
- Simultaneous identification of Mycobacterium genus and Mycobacterium tuberculosis complex in clinical samples by 5'-exonuclease fluorogenic PCR.Journal of clinical microbiology · 2002Article
- Detection of Mycobacterium avium in blood samples of patients with AIDS by using PCR.Journal of clinical microbiology · 2002Article
- Direct identification of bacteria from positive blood cultures by amplification and sequencing of the 16S rRNA gene: evaluation of BACTEC 9240 instrument true-positive and false-positive results.Journal of clinical microbiology · 2001Article
- Fluorescent In situ hybridization allows rapid identification of microorganisms in blood cultures.Journal of clinical microbiology · 2000Article
- Improved amplification of microbial DNA from blood cultures by removal of the PCR inhibitor sodium polyanetholesulfonate.Journal of clinical microbiology · 1998Article
- Detection and identification of mycobacteria by amplification of RNA and DNA in pretreated blood and bone marrow aspirates by a simple lysis method.Journal of clinical microbiology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A sodium iodide-isopropanol (NI) method was compared with an alkali wash and heat lysis (AH) procedure for the preparation and extraction of DNA from BACTEC 13A blood culture fluid samples from AIDS patients for use in a PCR for the detection and identification of mycobacteria. The sensitivity and efficiency of the DNA extraction methods were assessed by a multiplex PCR which detected the members of the genus Mycobacterium and differentiated between M. intracellulare, M. tuberculosis, and M. avium isolates with a limit of detection of between 0.28 pg (67 cells) and 120 pg (28,571 cells) of standard mycobacterial DNA. The PCR amplified mycobacterial DNA prepared by the AH procedure from 40 acid-fast bacillus-positive blood cultures with growth index values of > 20 U but not from 48 blood cultures with growth index values of < 21 U. The AH method was about 10 times more sensitive than the NI method for extracting DNA from 13 acid-fast bacillus-positive BACTEC fluid samples for PCR analysis. The study shows that the AH procedure in combination with the multiplex PCR is a simple, specific, and sensitive method which can be used in the routine diagnostic laboratory to detect and identify different members of the genus Mycobacterium in blood culture fluid samples from AIDS patients.
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