ArticleJournal of clinical microbiology1996
Rapid diagnosis of human parainfluenza virus type 1 infection by quantitative reverse transcription-PCR-enzyme hybridization assay.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 68 citations in OpenAlex.
- Clinical findings and unusual epidemiologic characteristics of human metapneumovirus infections in children in the region of Basel, Switzerland.European journal of pediatrics · 2008Trial
- Surveillance of Zoonotic Pathogens and Taxonomic Identification of Non-volant Small Mammals in Peninsular Malaysia.Tropical life sciences research · 2026Article
- Multiplex RT-PCR and indirect immunofluorescence assays for detection and subtyping of human influenza virus in Tunisia.Current microbiology · 2015Article
- Validation of a multiplex reverse transcriptase PCR ELISA for the detection of 19 respiratory tract pathogens.Infection · 2013Article
- Use and evaluation of molecular diagnostics for pneumonia etiology studies.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Review
- High viral load of human bocavirus correlates with duration of wheezing in children with severe lower respiratory tract infection.PloS one · 2012Article
- Epidemic viral pneumonia and other emerging pathogens.Clinics in chest medicine · 2011Review
- Impact of bacterial colonization on the severity, and accompanying airway inflammation, of virus-induced wheezing in children.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2010Article
- Respiratory viral infections detected by multiplex PCR among pediatric patients with lower respiratory tract infections seen at an urban hospital in Delhi from 2005 to 2007.Virology journal · 2009Article
- Simultaneous detection of parainfluenza viruses 1 and 3 by real-time reverse transcription-polymerase chain reaction.Journal of virological methods · 2009Article
- Article
- Detection of respiratory viruses by molecular methods.Clinical microbiology reviews · 2008Review
- Detection of 11 common viral and bacterial pathogens causing community-acquired pneumonia or sepsis in asymptomatic patients by using a multiplex reverse transcription-PCR assay with manual (enzyme hybridization) or automated (electronic microarray) detection.Journal of clinical microbiology · 2008Article
- Exposure to novel parainfluenza virus and clinical relevance in 2 bottlenose dolphin (Tursiops truncatus) populations.Emerging infectious diseases · 2008Article
- Detection and typing by molecular techniques of respiratory viruses in children hospitalized for acute respiratory infection in Rome, Italy.Journal of medical virology · 2007Article
- The cell biology of acute childhood respiratory disease: therapeutic implications.Pediatric clinics of North America · 2006Review
- Respiratory viral infections in patients with chronic, obstructive pulmonary disease.The Journal of infection · 2005Article
- The pneumoplex assays, a multiplex PCR-enzyme hybridization assay that allows simultaneous detection of five organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and its real-time counterpart.Journal of clinical microbiology · 2005Article
- Practical implementation of a multiplex PCR for acute respiratory tract infections in children.Journal of clinical microbiology · 2004Article
- Comparison of clinical characteristics of influenza and respiratory syncytial virus infection in hospitalised children and adolescents.European journal of pediatrics · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection and quantitation of human parainfluenza virus type 1 (HPIV-1) RNA in nasal wash specimens from 49 children with lower respiratory infections were performed by a reverse transcription-PCR-enzyme hybridization assay (RT-PCR-EHA). The HPIV-1 RT-PCR-EHA was then used to test 40 samples from asymptomatic children. Primers and probes were designed from regions within the HPIV-1 hemagglutinin-neuraminidase gene which are highly conserved among all known genotypes. HPIV-1 was detected in all nine children who were culture positive. Other common respiratory viruses (HPIV-2, -3, and -4, mumps virus, respiratory syncytial virus, and influenza virus) were not detected by the HPIV-1 assay. Forty symptomatic children were negative by culture, and four of these were positive by RT-PCR-EHA. All of the samples from asymptomatic children were negative by culture and RT-PCR-EHA. RT-PCR-EHA was 100% sensitive (95% confidence interval, 0.66 to 1.00) and 95% specific (95% confidence interval, 0.88 to 0.99) compared with culture. The four false-positive results (relative to the results of culture) were in children with lower respiratory infections compatible with HPIV-1 infection and suggest that RT-PCR-EHA may be more sensitive than culture. Our data indicate that HPIV-1 may be underdiagnosed by routine culturing methods. RT-PCR-EHA has been demonstrated to be an easy, rapid, sensitive, and specific test for diagnosing HPIV-1 infection and provides a methodology for the rapid detection of closely related respiratory viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.