ArticleMolecular biology of the cell1996
Overexpression of cytoplasmic dynein's globular head causes a collapse of the interphase microtubule network in Dictyostelium.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed.
- Katnip is needed to maintain microtubule function and lysosomal delivery to autophagosomes and phagosomes.Molecular biology of the cell · 2023Article
- TheCells · 2021Review
- Review
- Article
- Bioenergetics of theBiomolecules · 2020Article
- Review
- Emerging mechanisms of dynein transport in the cytoplasm versus the cilium.Biochemical Society transactions · 2018Review
- Organization of microtubule assemblies in Dictyostelium syncytia depends on the microtubule crosslinker, Ase1.Cellular and molecular life sciences : CMLS · 2016Article
- Mechanism and regulation of cytoplasmic dynein.Annual review of cell and developmental biology · 2015Review
- Functions and mechanics of dynein motor proteins.Nature reviews. Molecular cell biology · 2013Review
- A kinesin-mediated mechanism that couples centrosomes to nuclei.Cellular and molecular life sciences : CMLS · 2013Article
- Article
- X-ray structure of a functional full-length dynein motor domain.Nature structural & molecular biology · 2011Article
- Ciliary abnormalities due to defects in the retrograde transport protein DYNC2H1 in short-rib polydactyly syndrome.American journal of human genetics · 2009Article
- Motor- and tail-dependent targeting of dynein to microtubule plus ends and the cell cortex.Current biology : CB · 2009Article
- Article
- Disruption of four kinesin genes in dictyostelium.BMC cell biology · 2008Article
- The coordination of cyclic microtubule association/dissociation and tail swing of cytoplasmic dynein.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Two modes of microtubule sliding driven by cytoplasmic dynein.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Article
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2 authors.
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Abstract
Cytoplasmic dynein is a minus-end directed microtubule-based motor. Using a molecular genetic approach, we have begun to dissect structure-function relationships of dynein in the cellular slime mold Dictyostelium. Expression of a carboxy-terminal 380-kDa fragment of the heavy chain produces a protein that approximates the size and shape of the globular, mechanochemical head of dynein. This polypeptide cosediments with microtubules in an ATP-sensitive fashion and undergoes a UV-vanadate cleavage reaction. The deleted amino-terminal region appears to participate in dimerization of the native protein and in binding the intermediate and light chains. Overexpression of the 380-kDa carboxy-terminal construct in Dictyostelium produces a distinct phenotype in which the interphase radial microtubule array appears collapsed. In many cells, the microtubules form loose bundles that are whorled around the nucleus. Similar expression of a central 107-kDa fragment of the heavy chain does not produce this result. The data presented here suggest that dynein may participate in maintaining the spatial pattern of the interphase microtubule network.
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