ArticleMolecular biology of the cell1996
Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 75 citations in OpenAlex.
- Comprehensive pan-cancer evaluation of NUP85 prognosis, immune infiltration response, and validation in prostate cancer.Discover oncology · 2025Article
- Ablation of ZC3H11A causes early embryonic lethality and dysregulation of metabolic processes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Shaping the nucleus: factors and forces.Journal of cellular biochemistry · 2012Review
- The yeast nuclear pore complex and transport through it.Genetics · 2012Article
- Inner/Outer nuclear membrane fusion in nuclear pore assembly: biochemical demonstration and molecular analysis.Molecular biology of the cell · 2010Article
- Changes in the nuclear envelope environment affect spindle pole body duplication in Saccharomyces cerevisiae.Genetics · 2010Article
- Transportin regulates major mitotic assembly events: from spindle to nuclear pore assembly.Molecular biology of the cell · 2009Article
- ER membrane-bending proteins are necessary for de novo nuclear pore formation.The Journal of cell biology · 2009Article
- Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export.Molecular and cellular biology · 2008Article
- A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export.The EMBO journal · 2008Article
- The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes.The Journal of cell biology · 2007Article
- NUCLEOPORIN85 is required for calcium spiking, fungal and bacterial symbioses, and seed production in Lotus japonicus.The Plant cell · 2007Article
- The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly.Molecular biology of the cell · 2006Article
- Article
- Candida albicans biofilm-defective mutants.Eukaryotic cell · 2005Article
- Identification and biophysical characterization of a very-long-chain-fatty-acid-substituted phosphatidylinositol in yeast subcellular membranes.The Biochemical journal · 2004Article
- Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex.Molecular and cellular biology · 2003Article
- Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Binding dynamics of structural nucleoporins govern nuclear pore complex permeability and may mediate channel gating.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In a screen for mutants defective in nucleocytoplasmic export of mRNA, we have identified a new component of the Saccharomyces cerevisiae nuclear pore complex (NPC). The RAT9/NUP85 (ribonucleic acid trafficking) gene encodes an 84.9-kDa protein that we have localized to NPCs by tagging the RAT9/NUP85 gene with the in vivo molecular marker Green Fluorescent Protein. In cells containing either the rat9-1 allele or a complete deletion of the RAT9/NUP85 gene, poly(A)+ RNA accumulates rapidly in nuclei after a shift from 23 degrees C to 37 degrees C. Under these same conditions, rapid fragmentation of the nucleolus was also observed. At the permissive growth temperature in rat9-1 or RAT9 deletion strains, the nuclear envelope (NE) becomes detached from the main body of the nucleus, forming long thin double sheets of NE. NPCs within these sheets are clustered and some appear to be locked together between opposing sheets of NE such that their nucleoplasmic faces are in contact. The Rat9/Nup85 protein could not be detected in cells carrying a mutation of RAT2/NUP120, suggesting that Rat9p/Nup85p cannot be assembled into NPCs in the absence of Rat2p/Nup120p. In contrast,Rat9/ Nup85 protein was readily incorporated into NPCs in strains carrying mutant alleles of other nucleoporin genes. The possible role of Rat9p/Nup85p in NE integrity and the loss of nucleoporins when another nucleoporin is mutant or absent are discussed.
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Registered trials
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