ArticleMolecular biology of the cell1996
The pool of map kinase associated with microtubules is small but constitutively active.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 142 citations in OpenAlex.
- Nucleus Accumbens Proteome Disbalance in an Adolescent Mouse Model of Schizophrenia and Nicotine Misuse Comorbidity.Biomedicines · 2025Article
- Nicotine Exposure in a Phencyclidine-Induced Mice Model of Schizophrenia: Sex-Selective Medial Prefrontal Cortex Protein Markers of the Combined Insults in Adolescent Mice.International journal of molecular sciences · 2023Article
- Microtubules in insulin action: what's on the tube?Trends in endocrinology and metabolism: TEM · 2021Review
- PHF-Core Tau as the Potential Initiating Event for Tau Pathology in Alzheimer's Disease.Frontiers in cellular neuroscience · 2020Article
- Genome-Wide Screen for New Components of theG3 (Bethesda, Md.) · 2018Article
- Regulatory Roles of Fluctuation-Driven Mechanotransduction in Cell Function.Physiology (Bethesda, Md.) · 2016Review
- Focusing on the focus: what else beyond the master switches for polar cell growth?Molecular plant · 2015Review
- Transport and diffusion of Tau protein in neurons.Cellular and molecular life sciences : CMLS · 2014Review
- Arrestin-dependent activation of JNK family kinases.Handbook of experimental pharmacology · 2014Article
- Epidermal growth factor stimulates extracellular-signal regulated kinase phosphorylation of a novel site on cytoplasmic Dynein intermediate chain 2.International journal of molecular sciences · 2013Article
- Biochemistry and cell biology of tau protein in neurofibrillary degeneration.Cold Spring Harbor perspectives in medicine · 2012Review
- Reactive oxygen species, Ki-Ras, and mitochondrial superoxide dismutase cooperate in nerve growth factor-induced differentiation of PC12 cells.The Journal of biological chemistry · 2010Article
- Heparin-binding epidermal growth factor-like growth factor is a potent neurotrophic factor for PC12 cells.Neuro-Signals · 2010Article
- Protein scaffolds in MAP kinase signalling.Cellular signalling · 2009Review
- Identification and specific localization of tyrosine-phosphorylated proteins in Trypanosoma brucei.Eukaryotic cell · 2009Article
- Changes in apical dendritic structure correlate with sustained ERK1/2 phosphorylation in medial prefrontal cortex of a rat model of dopamine D1 receptor agonist sensitization.The Journal of comparative neurology · 2008Article
- Cdk5 Modulation of mitogen-activated protein kinase signaling regulates neuronal survival.Molecular biology of the cell · 2007Article
- Distinct signalling particles containing ERK/MEK and B-Raf in PC12 cells.The Biochemical journal · 2005Article
- Transgenic animal models of Alzheimer's disease and related disorders: histopathology, behavior and therapy.Molecular psychiatry · 2004Review
- Over-expression of the mitogen-activated protein kinase (MAPK) kinase (MEK)-MAPK in hepatocellular carcinoma: its role in tumor progression and apoptosis.BMC gastroenterology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitogen-activated protein kinase (MAPK) is activated by many kinds of stimuli and plays an important role in integrating signal transduction cascades. MAPK is present abundantly in brain, where we have studied its association with microtubules. Immunofluorescence of primary hippocampal neurons revealed that MAPK staining co-localized with microtubules and biochemical analyses showed that MAPK co-purified with microtubules. Approximately 4% of MAPK in cytosolic extracts was associated with microtubules, where it was associated with both tubulin and microtubule-associated proteins (MAPs) fractions. Further fractionation of MAPs suggested that a portion of MAPK is associated with MAP2. An association with MAP2 was also demonstrated by co-immunoprecipitation and in vitro binding experiments. A similar association was shown for the juvenile MAP2 isoform, MAP2C. The pool of MAPK associated with microtubules had a higher activity relative to the nonassociated pool in both brain and proliferating PC12 cells. Although MAPK was activated by nerve growth factor in PC12 cells, the activity of microtubule-associated MAPK did not further increase. These results raise the possibility that microtubule-associated MAPK operates through constitutive phosphorylation activity to regulate microtubule function in neurons.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.