ArticleProceedings of the National Academy of Sciences of the United States of America1996
Mutations in the small subunit of the Drosophila U2AF splicing factor cause lethality and developmental defects.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 70 citations in OpenAlex.
- U2af1 is a haplo-essential gene required for hematopoietic cancer cell survival in mice.The Journal of clinical investigation · 2021Article
- Splice site mCell · 2021Article
- Article
- Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons.Nucleic acids research · 2017Article
- Unmasking the U2AF homology motif family: a bona fide protein-protein interaction motif in disguise.RNA (New York, N.Y.) · 2016Review
- Recognition of the 3' splice site RNA by the U2AF heterodimer involves a dynamic population shift.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- RNA structure in splicing: An evolutionary perspective.RNA biology · 2016Review
- Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins.Nucleic acids research · 2015Article
- Genomic functions of U2AF in constitutive and regulated splicing.RNA biology · 2015Article
- Recurrent mutations in the U2AF1 splicing factor in myelodysplastic syndromes.Nature genetics · 2011Article
- The translation initiation factor eIF4E regulates the sex-specific expression of the master switch gene Sxl in Drosophila melanogaster.PLoS genetics · 2011Article
- Genomic mRNA profiling reveals compensatory mechanisms for the requirement of the essential splicing factor U2AF.Molecular and cellular biology · 2011Article
- Mutations in the Caenorhabditis elegans U2AF large subunit UAF-1 alter the choice of a 3' splice site in vivo.PLoS genetics · 2009Article
- Functioning of the Drosophila Wilms'-tumor-1-associated protein homolog, Fl(2)d, in Sex-lethal-dependent alternative splicing.Genetics · 2008Article
- A conditional role of U2AF in splicing of introns with unconventional polypyrimidine tracts.Molecular and cellular biology · 2007Article
- The impact of alternative splicing in vivo: mouse models show the way.RNA (New York, N.Y.) · 2007Review
- RNA interference knockdown of hU2AF35 impairs cell cycle progression and modulates alternative splicing of Cdc25 transcripts.Molecular biology of the cell · 2006Article
- Molecular characterization and phylogeny of U2AF35 homologs in plants.Plant physiology · 2006Article
- Human splicing factor SF3a, but not SF1, is essential for pre-mRNA splicing in vivo.Molecular biology of the cell · 2005Article
- Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.Molecular biology of the cell · 2005Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The essential eukaryotic pre-mRNA splicing factor U2AF (U2 small nuclear ribonucleoprotein auxiliary factor) is required to specify the 3' splice at an early step in spliceosome assembly. U2AF binds site-specifically to the intron polypyrimidine tract and recruits U2 small nuclear ribonucleoprotein to the branch site. Human U2AF (hU2AF) is a heterodimer composed of a large (hU2AF65) and small (hU2AF35) subunit. Although these proteins associate in a tight complex, the biochemical requirement for U2AF activity can be satisfied solely by the large subunit. The requirement for the small subunit in splicing has remained enigmatic. No biochemical activity has been found for hU2AF35 and it has been implicated in splicing only indirectly by its interaction with known splicing factors. In the absence of a biochemical assay, we have taken a genetic approach to investigate the function of the small subunit in the fruit fly Drosophila melanogaster. A cDNA clone encoding the small subunit of Drosophila U2AF (dU2AF38) has been isolated and sequenced. The dU2AF38 protein is highly homologous to hU2AF35 containing a conserved central arginine- and serine-rich (RS) domain. A recessive P-element insertion mutation affecting dU2AF38 causes a reduction in viability and fertility and morphological bristle defects. Consistent with a general role in splicing, a null allele of dU2AF38 is fully penetrant recessive lethal, like null alleles of the Drosophila U2AF large subunit.
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