ArticleMolecular and cellular biology1996
Transcriptional regulation by ergosterol in the yeast Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 92 citations in OpenAlex.
- Article
- Integrated omics of Saccharomyces cerevisiae CENPK2-1C reveals pleiotropic drug resistance and lipidomic adaptations to cannabidiol.NPJ systems biology and applications · 2024Article
- Amphotericin B resistance in Leishmania mexicana: Alterations to sterol metabolism and oxidative stress response.PLoS neglected tropical diseases · 2022Article
- Influence of ergosterol and phytosterols on wine alcoholic fermentation withFrontiers in microbiology · 2022Article
- Learning causal networks using inducible transcription factors and transcriptome-wide time series.Molecular systems biology · 2020Article
- Modular 5'-UTR hexamers for context-independent tuning of protein expression in eukaryotes.Nucleic acids research · 2018Article
- Quantitative Profiling of N-linked Glycosylation Machinery in YeastMolecular & cellular proteomics : MCP · 2018Article
- Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae.Microbial cell factories · 2015Article
- UPC2A is required for high-level azole antifungal resistance in Candida glabrata.Antimicrobial agents and chemotherapy · 2014Article
- The yeast anaerobic response element AR1b regulates aerobic antifungal drug-dependent sterol gene expression.The Journal of biological chemistry · 2013Article
- Trans-chalcone and quercetin down-regulate fatty acid synthase gene expression and reduce ergosterol content in the human pathogenic dermatophyte Trichophyton rubrum.BMC complementary and alternative medicine · 2013Article
- PP2A(Cdc55) regulates G1 cyclin stability.Cell cycle (Georgetown, Tex.) · 2013Article
- Beyond de-foaming: the effects of antifoams on bioprocess productivity.Computational and structural biotechnology journal · 2012Review
- Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures.FEMS yeast research · 2011Article
- Development of a novel multiplex DNA microarray for Fusarium graminearum and analysis of azole fungicide responses.BMC genomics · 2011Article
- Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae.Fungal genetics and biology : FG & B · 2008Article
- Reduced susceptibility to polyenes associated with a missense mutation in the ERG6 gene in a clinical isolate of Candida glabrata with pseudohyphal growth.Antimicrobial agents and chemotherapy · 2007Article
- Article
- Dual activators of the sterol biosynthetic pathway of Saccharomyces cerevisiae: similar activation/regulatory domains but different response mechanisms.Molecular and cellular biology · 2005Article
- Enriching for direct regulatory targets in perturbed gene-expression profiles.Genome biology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Sterol biosynthesis in the yeast Saccharomyces cerevisiae is an energy-expensive, aerobic process, requiring heme and molecular oxygen. Heme, also synthesized exclusively during aerobic growth, not only acts as an enzymatic cofactor but also is directly and indirectly responsible for the transcriptional control of several yeast genes. Because of their biosynthetic similarities, we hypothesized that ergosterol, like heme, may have a regulatory function. Sterols are known to play a structural role in membrane integrity, but regulatory roles have not been characterized. To test possible regulatory roles of sterol, the promoter for the ERG3 gene, encoding the sterol C-5 desaturase, was fused to the bacterial lacZ reporter gene. This construct was placed in strains making aberrant sterols, and the effect of altered sterol composition on gene expression was monitored by beta-galactosidase activity. The absence of ergosterol resulted in a 35-fold increase in the expression of ERG3 as measured by beta-galactosidase activity. The level of ERG3 mRNA was increased as much as ninefold in erg mutant strains or wild-type strains inhibited in ergosterol biosynthesis by antifungal agents. The observed regulatory effects of ergosterol on ERG3 are specific for ergosterol, as several ergosterol derivatives failed to elicit the same controlling effect. These results demonstrate for the first time that ergosterol exerts a regulatory effect on gene transcription in S. cerevisiae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.