ArticleMolecular and cellular biology1996
High-resolution mapping of the origin of DNA replication in the hamster dihydrofolate reductase gene domain by competitive PCR.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 80 citations in OpenAlex.
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- Detection of slipped-DNAs at the trinucleotide repeats of the myotonic dystrophy type I disease locus in patient tissues.PLoS genetics · 2013Article
- Initiation of DNA Replication in the Human Genome.Hereditary genetics : current research · 2012Article
- Genomic study of replication initiation in human chromosomes reveals the influence of transcription regulation and chromatin structure on origin selection.Molecular biology of the cell · 2010Article
- A winding road to origin discovery.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Isolation of restriction fragments containing origins of replication from complex genomes.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Overreplication of short DNA regions during S phase in human cells.Genes & development · 2008Article
- Specific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication.Genes & development · 2005Article
- Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin.Molecular and cellular biology · 2004Article
- The promoter of the Chinese hamster ovary dihydrofolate reductase gene regulates the activity of the local origin and helps define its boundaries.Genes & development · 2004Article
- The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The dihydrofolate reductase origin of replication does not contain any nonredundant genetic elements required for origin activity.Molecular and cellular biology · 2003Article
- Developmental changes in the Sciara II/9A initiation zone for DNA replication.Molecular and cellular biology · 2002Article
- Initiation sites are distributed at frequent intervals in the Chinese hamster dihydrofolate reductase origin of replication but are used with very different efficiencies.Molecular and cellular biology · 2002Article
- Mammalian Orc1 protein is selectively released from chromatin and ubiquitinated during the S-to-M transition in the cell division cycle.Molecular and cellular biology · 2002Article
- Making sense of eukaryotic DNA replication origins.Science (New York, N.Y.) · 2001Review
- Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycle.Nucleic acids research · 2001Article
- The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.Molecular and cellular biology · 2001Article
- Multiple sites of replication initiation in the human beta-globin gene locus.Nucleic acids research · 2001Article
- Enhanced flexibility and aphidicolin-induced DNA breaks near mammalian replication origins: implications for replicon mapping and chromosome fragility.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By the use of a highly sensitive mapping procedure allowing the identification of the start sites of DNA replication in single-copy genomic regions of untreated, exponentially growing cultured cells (M. Giacca, L. Zentilin, P. Norio, S. Diviacco, D. Dimitrova, G. Contreas, G. Biamonti, G. Perini, F. Weighardt, S. Riva, and A. Falaschi, Proc. Natl. Acad. Sci. USA 91:7119-7123, 1994), the pattern of DNA replication of the Chinese hamster dihydrofolate reductase (DHFR) gene domain was investigated. The method entails the purification of short stretches of nascent DNA issuing from DNA replication origin regions and quantification, within this sample, of the abundance of different adjacent segments by competitive PCR. Distribution of marker abundance peaks around the site from which newly synthesized DNA had emanated. The results obtained by analysis of the genomic region downstream of the DHFR single-copy gene in asynchronous cultures of hamster CHO K1 cells are consistent with the presence of a single start site for DNA replication, located approximately 17 kb downstream of the gene. This site is coincident with the one detected by other studies using different techniques in CHO cell lines containing an amplified DHFR gene domain.
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