ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1996
Activity-independent segregation of excitatory and inhibitory synaptic terminals in cultured hippocampal neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 67 citations in OpenAlex.
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- Activity Dependent and Independent Determinants of Synaptic Size Diversity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Co-activation of selective nicotinic acetylcholine receptors is required to reverse beta amyloid-induced CaNeurobiology of aging · 2019Article
- Modeling a Neurexin-3α Human Mutation in Mouse Neurons Identifies a Novel Role in the Regulation of Transsynaptic Signaling and Neurotransmitter Release at Excitatory Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Calcium imaging, MEA recordings, and immunostaining images dataset of neuron-astrocyte networks in culture under the effect of norepinephrine.GigaScience · 2019Article
- Theta rhythm-like bidirectional cycling dynamics of living neuronal networks in vitro.PloS one · 2018Article
- Rewiring of neuronal networks during synaptic silencing.Scientific reports · 2017Article
- SRGAP2 and Its Human-Specific Paralog Co-Regulate the Development of Excitatory and Inhibitory Synapses.Neuron · 2016Article
- Immunohistochemical and ultrastructural properties of the larval ciliary band-associated strand in the sea urchin Hemicentrotus pulcherrimus.Frontiers in zoology · 2016Article
- Ontogeny of biochemical, morphological and functional parameters of synaptogenesis in primary cultures of rat hippocampal and cortical neurons.Molecular brain · 2015Article
- β1-Integrin cytoskeletal signaling regulates sensory neuron response to matrix dimensionality.Neuroscience · 2013Article
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- Substrate three-dimensionality induces elemental morphological transformation of sensory neurons on a physiologic timescale.Tissue engineering. Part A · 2012Article
- Coordinated increase in inhibitory and excitatory synapses onto retinal ganglion cells during development.Neural development · 2011Article
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- Development of input connections in neural cultures.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Development of gamma-aminobutyric acidergic synapses in cultured hippocampal neurons.The Journal of comparative neurology · 2006Article
- Synaptic vesicle recycling studied in transgenic mice expressing synaptopHluorin.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cultured hippocampal neurons were used as a model system to address experimentally the spatial and temporal sequence leading to the appropriate sorting of excitatory and inhibitory synaptic terminals to different cellular target domains and the role of neural activity in this process. By using antibodies against glutamic acid decarboxylase 65 (GAD65) and synaptophysin, we examined the development and segregation of GABAergic and non-GABAergic synaptic terminals on single neurons. Electron microscopy confirmed that GAD65-labeled swellings observed using light microscopy corresponded to synaptic boutons. From the time at which GABAergic terminals first appeared, they developed at a more rapid rate on neuronal somata than non-GABAergic terminals did, such that by 18 d in culture, 60% of the total boutons on somata were GABAergic. By contrast, the majority (70%) of boutons on dendrites were non-GABAergic. These data suggest that inhibitory synaptic terminals are targeted preferentially to or maintained on cell somata at the expense of excitatory terminals. Interestingly, non-GABAergic terminals were not inhibited from forming synapses on cell somata, because in the absence of GABAergic terminals they attained the same total somatic terminal density seen in the presence of GABAergic terminals. Chronic blockade of neuronal activity did not affect the differential targeting of GABAergic and non-GABAergic axons; however, it did reduce the extent of dendritic arborization. Our findings support a two-step model for synaptic segregation whereby the majority of terminals is initially targeted in an activity-independent manner to the appropriate cellular domains, but an additional developmental mechanism serves to further restrict and refine the original synaptic distribution.
Indexed as
Identifiers
8815921PMC6578921W1935595667What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.