ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1996
Hair cell differentiation in chick cochlear epithelium after aminoglycoside toxicity: in vivo and in vitro observations.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
28 citing papers in PubMed, 91 citations in OpenAlex.
- A comparative single-cell transcriptomic atlas for diverse populations of vertebrate hair cells.bioRxiv : the preprint server for biology · 2026Article
- Single-Cell Landscape of the Cochlea Revealed Cell-Type-Specific Diversification inBiomolecules · 2025Article
- A NovelFrontiers in molecular neuroscience · 2021Article
- Perspectives on Human Hearing Loss, Cochlear Regeneration, and the Potential for Hearing Restoration Therapies.Brain sciences · 2020Review
- Initiation of Supporting Cell Activation for Hair Cell Regeneration in the Avian Auditory Epithelium: An Explant Culture Model.Frontiers in cellular neuroscience · 2020Article
- Study of the Mechanisms by Which Aminoglycoside Damage Is Prevented in Chick Embryonic Hair Cells.Journal of the Association for Research in Otolaryngology : JARO · 2019Article
- Bone morphogenetic protein 4 antagonizes hair cell regeneration in the avian auditory epithelium.Hearing research · 2018Article
- A brief history of hair cell regeneration research and speculations on the future.Hearing research · 2013Review
- Depletion of resident macrophages does not alter sensory regeneration in the avian cochlea.PloS one · 2012Article
- Transcriptomic analysis of the zebrafish inner ear points to growth hormone mediated regeneration following acoustic trauma.BMC neuroscience · 2011Article
- Comparative analysis of combination kanamycin-furosemide versus kanamycin alone in the mouse cochlea.Hearing research · 2011Article
- Supporting cell division is not required for regeneration of auditory hair cells after ototoxic injury in vitro.Journal of the Association for Research in Otolaryngology : JARO · 2010Article
- Activin potentiates proliferation in mature avian auditory sensory epithelium.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Feathers and fins: non-mammalian models for hair cell regeneration.Brain research · 2009Review
- Notch regulation of progenitor cell behavior in quiescent and regenerating auditory epithelium of mature birds.Developmental biology · 2009Article
- Peptide- and collagen-based hydrogel substrates for in vitro culture of chick cochleae.Biomaterials · 2008Article
- Closure of supporting cell scar formations requires dynamic actin mechanisms.Hearing research · 2007Article
- Neomycin-induced hair cell death and rapid regeneration in the lateral line of zebrafish (Danio rerio).Journal of the Association for Research in Otolaryngology : JARO · 2003Article
- Cell density and N-cadherin interactions regulate cell proliferation in the sensory epithelia of the inner ear.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Inhibition of caspases prevents ototoxic and ongoing hair cell death.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Inner ear epithelia of mature birds regenerate hair cells after ototoxic or acoustic insult. The lack of markers that selectively label cells in regenerating epithelia and of culture systems composed primarily of progenitor cells has hampered the identification of cellular and molecular interactions that regulate hair cell regeneration. In control basilar papillae, we identified two markers that selectively label hair cells (calmodulin and TUJ1 beta tubulin antibodies) and one marker unique for support cells (cytokeratin antibodies). Examination of regenerating epithelia demonstrated that calmodulin and beta tubulin are also expressed in early differentiating hair cells, and cytokeratins are retained in proliferative support cells. Enzymatic and mechanical methods were used to isolate sensory epithelia from mature chick basilar papillae, and epithelia were cultured in different conditions. In control cultures, hair cells are morphologically stable for up to 6 d, because calmodulin immunoreactivity and phalloidin labeling of filamentous actin are retained. The addition of an ototoxic antibiotic to cultures, however, causes complete hair cell loss by 2 d in vitro and generates cultures composed of calmodulin-negative, cytokeratin-positive support cells. These cells are highly proliferative for the first 2-7 d after plating, but stop dividing by 9 d. Calmodulin- or TUJ1-positive cells reemerge in cultures treated with antibiotic for 5 d and maintained for an additional 5 d without antibiotic. A subset of calmodulin-positive cells was also labeled with BrdU when it was continuously present in cultures, suggesting that some cells generated in culture begin to differentiate into hair cells.
Indexed as
Identifiers
8815898PMC6579194W2154409385What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.