ArticleJournal of clinical microbiology1996
Host DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 80 citations in OpenAlex.
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- Detection and quantification of Erysipelothrix rhusiopathiae in blood from infected chickens - addressing challenges with detection of DNA from infectious agents in host species with nucleated red blood cells.Journal of medical microbiology · 2019Article
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- Targeted Treatment for Bacterial Infections: Prospects for Pathogen-Specific Antibiotics Coupled with Rapid Diagnostics.Tetrahedron · 2016Article
- Visual Detection of Brucella spp. in Spiked Bovine Semen Using Loop-Mediated Isothermal Amplification (LAMP) Assay.Indian journal of microbiology · 2016Article
- Improved sensitivity for molecular detection of bacterial and Candida infections in blood.Journal of clinical microbiology · 2014Article
- DNA sequencing diagnosis of off-season spirochetemia with low bacterial density in Borrelia burgdorferi and Borrelia miyamotoi infections.International journal of molecular sciences · 2014Article
- Article
- The application of multiplex PCR to detect seven different DNA targets in group B streptococci.Folia microbiologica · 2012Article
- Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption.Journal of microbiological methods · 2011Article
- Cryptic vector divergence masks vector-specific patterns of infection: an example from the marine cycle of Lyme borreliosis.Evolutionary applications · 2010Article
- Diagnosis of canine brucellosis: comparison between serological and microbiological tests and a PCR based on primers to 16S-23S rDNA interspacer.Veterinary research communications · 2007Article
- Quantitative detection of Staphylococcus aureus and Enterococcus faecalis DNA in blood to diagnose bacteremia in patients in the intensive care unit.Journal of clinical microbiology · 2007Article
- Diagnosis of lyme borreliosis.Clinical microbiology reviews · 2005Review
- Improved method of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical samples by polymerase chain reaction without DNA purification.Folia microbiologica · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
It is demonstrated that a diagnostic PCR for Borrelia burgdorferi can be inhibited in the presence of more than 500 ng of host (monkey skin) DNA. The inhibitor is the host DNA itself. An acceptable value for analytical sensitivity can be obtained by diluting the skin-B. burgdorferi proteinase K lysate to a level below the inhibitory concentration of the host DNA. Dilution of the lysate may obviate the need for further DNA purification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.