ArticleJournal of clinical microbiology1996
Diagnostic performance of amplified Mycobacterium tuberculosis direct test with cerebrospinal fluid, other nonrespiratory, and respiratory specimens.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 2 syntheses or guidelines pooled it, 169 citations in OpenAlex.
- Pooled it
- Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis.BMC infectious diseases · 2004Pooled it
- Primary Tubercular Abscess of the Axilla-a Rare Case.The Indian journal of surgery · 2017Article
- Rapid diagnosis of Mycobacterium tuberculosis with Truenat MTB: a near-care approach.PloS one · 2013Article
- Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities.Clinical microbiology reviews · 2011Review
- Rapid identification of mycobacterium species with the aid of multiplex polymerase chain reaction (PCR) from clinical isolates.The open microbiology journal · 2010Article
- Clinical evaluation of TRCRapid M.TB for detection of Mycobacterium tuberculosis complex in respiratory and nonrespiratory specimens.Journal of clinical microbiology · 2010Article
- Molecular diagnostics in tuberculosis: basis and implications for therapy.Molecular diagnosis & therapy · 2009Review
- Rapid etiological classification of meningitis by NMR spectroscopy based on metabolite profiles and host response.PloS one · 2009Article
- Evaluation of the results of Mycobacterium tuberculosis direct test (MTD) and Mycobacterial culture in urine samples.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2008Article
- Central nervous system tuberculosis: pathogenesis and clinical aspects.Clinical microbiology reviews · 2008Review
- Multidrug-resistant tuberculous meningitis.Current neurology and neuroscience reports · 2007Review
- Mycobacterium tuberculosis and rifampin resistance, United Kingdom.Emerging infectious diseases · 2006Article
- False-positive amplified Mycobacterium tuberculosis direct test results for samples containing Mycobacterium leprae.Journal of clinical microbiology · 2006Article
- Molecular diagnostics in tuberculosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2005Review
- Diagnosis of extrapulmonary tuberculosis by smear, culture, and PCR using universal sample processing technology.Journal of clinical microbiology · 2005Article
- Comparison of the COBAS AMPLICOR MTB and BDProbeTec ET assays for detection of Mycobacterium tuberculosis in respiratory specimens.Journal of clinical microbiology · 2005Article
- Evaluation of a modified gen-probe amplified direct test for detection of Mycobacterium tuberculosis complex organisms in cerebrospinal fluid.Journal of clinical microbiology · 2004Article
- Improved sensitivity of nucleic acid amplification for rapid diagnosis of tuberculous meningitis.Journal of clinical microbiology · 2004Article
- Comparison of conventional bacteriology with nucleic acid amplification (amplified mycobacterium direct test) for diagnosis of tuberculous meningitis before and after inception of antituberculosis chemotherapy.Journal of clinical microbiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Gen-Probe Amplified Mycobacterium tuberculosis Direct Test (MTD) was adapted to be used for cerebrospinal fluid (CSF) and a large variety of other nonrespiratory specimens. Standardized with artificially spiked dilution series of CSF, the modified MTD procedure consists of (i) increasing the amount of sample 10-fold, (ii) pretreating the specimen with a detergent, and (iii) increasing the amplification time from 2 to 3 h. Performance of MTD in a clinical mycobacteriology laboratory was tested over an extended period of time, involving a total of 322 nonrespiratory as well as 1,117 respiratory specimens from 998 patients. Results from MTD were compared with those from microscopy, culture, analysis of tuberculostearic acid by gas-liquid chromatography-mass spectrometry (CSF only), and the final clinical diagnosis. When MTD results were compared with resolved data, the sensitivity, specificity, and positive and negative predictive values for MTD were 93.1, 97.7, 90.0, and 98.5%, respectively, for nonrespiratory specimens and 86.6, 96.4, 76.8, and 98.1%, respectively, for respiratory specimens. Our data demonstrate that (i) MTD is a robust, highly sensitive and specific technique for the rapid detection of M. tuberculosis complex in all types of clinical specimens, (ii) there was no statistically significant difference (P > 0.005) in sensitivity and specificity for nonrespiratory compared with respiratory specimens, and (iii) repeating all MTDs which yield a result between 30,000 and 200,000 relative light units would help prevent a large number of false positives and, thus, enhance test specificity.
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