ArticleThe Journal of physiology1996
Cooling inhibits exocytosis in single mouse pancreatic B-cells by suppression of granule mobilization.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 103 citations in OpenAlex.
- tPA-GFP is a reliable probe for detecting compound exocytosis in human pancreatic β-cells.FASEB bioAdvances · 2025Article
- Proinsulin folding and trafficking defects trigger a common pathological disturbance of endoplasmic reticulum homeostasis.Protein science : a publication of the Protein Society · 2024Article
- Serum potassium changes during hypothermia and rewarming: a case series and hypothesis on the mechanism.Clinical kidney journal · 2023Article
- A critical role of the mechanosensor PIEZO1 in glucose-induced insulin secretion in pancreatic β-cells.Nature communications · 2022Article
- Secretory granule exocytosis and its amplification by cAMP in pancreatic β-cells.Diabetology international · 2022Review
- Temperature effects on synaptic transmission and neuronal function in the visual thalamus.PloS one · 2020Article
- Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men.Physiological reviews · 2018Review
- The Prohormone VGF Regulates β Cell Function via Insulin Secretory Granule Biogenesis.Cell reports · 2017Article
- Lessons from basic pancreatic beta cell research in type-2 diabetes and vascular complications.Diabetology international · 2017Article
- Modulation of microRNA-375 expression alters voltage-gated Na(+) channel properties and exocytosis in insulin-secreting cells.Acta physiologica (Oxford, England) · 2015Article
- Cooling reduces cAMP-stimulated exocytosis and adiponectin secretion at a Ca2+-dependent step in 3T3-L1 adipocytes.PloS one · 2015Article
- Preconditioning with associated blocking of Ca2+ inflow alleviates hypoxia-induced damage to pancreatic β-cells.PloS one · 2013Article
- Characteristics and functions of {alpha}-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors expressed in mouse pancreatic {alpha}-cells.Endocrinology · 2010Article
- Exocytosis mechanisms underlying insulin release and glucose uptake: conserved roles for Munc18c and syntaxin 4.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Review
- Mechanisms of biphasic insulin-granule exocytosis - roles of the cytoskeleton, small GTPases and SNARE proteins.Journal of cell science · 2009Review
- Temperature enhances exocytosis efficiency at the mouse inner hair cell ribbon synapse.The Journal of physiology · 2007Article
- Physiological temperatures reduce the rate of vesicle pool depletion and short-term depression via an acceleration of vesicle recruitment.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Membrane reserves and hypotonic cell swelling.The Journal of membrane biology · 2006Article
- Maintenance of stimulus-secretion coupling and single beta-cell function in cryopreserved-thawed human islets of Langerhans.Pflugers Archiv : European journal of physiology · 2005Article
- CaV2.3 calcium channels control second-phase insulin release.The Journal of clinical investigation · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The mechanisms by which cooling inhibits insulin secretion were investigated by capacitance measurements of exocytosis in single mouse pancreatic B-cells maintained in short-term tissue culture. 2. A reduction of the bath temperature from 34 to 24 degrees C produced a gradual inhibition of exocytosis. Inhibition of exocytosis was use dependent rather than time dependent. The steady-state inhibition amounted to 90%, which was paralleled by a 30% reduction of the peak Ca2+ current. 3. The Q10 values (between 27 and 37 degrees C) for inhibition of exocytosis and the peak Ca2+ current amplitude were determined as > 5 and 1.6, respectively. From the temperature dependence of exocytosis, an energy of activation was estimated as 145 kJ mol-1. 4. Suppression of exocytosis was not the result of a reduction of Ca2+ influx. When the Ca2+ currents were blocked by 30% (comparable to that produced by cooling) by using a low concentration of Co2+, exocytosis was reduced by < 25%. 5. Elevation of cytoplasmic free Ca2+, by photorelease of 'caged' Ca2+ from Ca(2+)-nitrophenyl-EGTA preloaded into the B-cell, was equally effective at eliciting exocytosis at 24 and 34 degrees C. 6. Cooling produced 70% inhibition of exocytosis evoked by infusion of Ca2+ through the recording electrode. Omission of either MgATP or cAMP from the electrode solution resulted in a comparable reduction of exocytosis. Cooling had no additional inhibitory effect when exocytosis was already suppressed by removal of cytoplasmic MgATP. 7. Our data indicate that exocytosis of granules already docked beneath the membrane is little affected by cooling in the B-cell. Instead, the high overall temperature sensitivity of insulin secretion arises because the replenishment of the readily releasable pool is temperature dependent.
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