ArticleThe Journal of physiology1996
P2 purinoceptor-activated inward currents in follicular oocytes of Xenopus laevis.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 53 citations in OpenAlex.
- Purinergic signaling pathways in endocrine system.Autonomic neuroscience : basic & clinical · 2015Review
- Purinergic signalling in endocrine organs.Purinergic signalling · 2014Review
- Purinergic signalling in the reproductive system in health and disease.Purinergic signalling · 2014Review
- Tryptophan 46 is a site for ethanol and ivermectin action in P2X4 receptors.Purinergic signalling · 2013Article
- Roles of ectodomain and transmembrane regions in ethanol and agonist action in purinergic P2X2 and P2X3 receptors.Neuropharmacology · 2008Article
- Stimulation of rat erythrocyte P2X7 receptor induces the release of epoxyeicosatrienoic acids.British journal of pharmacology · 2007Article
- Antagonism of ATP responses at P2X receptor subtypes by the pH indicator dye, Phenol red.British journal of pharmacology · 2005Article
- Extended pharmacological profiles of rat P2Y2 and rat P2Y4 receptors and their sensitivity to extracellular H+ and Zn2+ ions.British journal of pharmacology · 2003Article
- Release of ATP induced by hypertonic solutions in Xenopus oocytes.The Journal of physiology · 2003Article
- Heteromultimeric P2X(1/2) receptors show a novel sensitivity to extracellular pH.The Journal of pharmacology and experimental therapeutics · 2002Article
- Actions of a Series of PPADS Analogs at P2XDrug development research · 2001Article
- Coexpression of rat P2X2 and P2X6 subunits in Xenopus oocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- A pyridoxine cyclic phosphate and its 6-azoaryl derivative selectively potentiate and antagonize activation of P2X1 receptors.Journal of medicinal chemistry · 1998Article
- Selectivity and activity of adenine dinucleotides at recombinant P2X2 and P2Y1 purinoceptors.British journal of pharmacology · 1996Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
1. ATP, UTP and 2MeSATP (10 microM) evoked fast, transient (2-20 s duration) and slow, sustained (60-120 s duration) inward currents in 118 of 285 follicular Xenopus oocytes but failed to activate inward currents in defolliculated Xenopus oocytes (n = 136). 2. The potency order for nucleotides was (at 10 microM): UTP = 2MeSATP > 2ClATP = ATP > ADP > alpha, beta-methylene-ATP > adenosine > ITP, with beta, gamma-methylene-ATP, CTP and GTP inactive. This potency order is atypical for any single P2 purinoceptor subtype. 3. EC50 values (for fast and slow inward currents) were (microM): ATP, 5.6 +/- 2.1 and 6.7 +/- 3.0; UTP, 1.0 +/- 0.3 and 0.9 +/- 0.3; 2MeSATP, 0.6 +/- 0.2 and 9.2 +/- 7.4 (n = 3). Suramin inhibited fast (IC50: 65 +/- 20 microM; n = 7) and slow (IC50: 57 +/- 10 microM; n = 7) inward currents evoked by ATP. Suramin also inhibited slow inward currents produced by UTP but potentiated those caused by 2MeSATP. 4. Fast and slow inward currents evoked in response to ATP and UTP reversed at -12 +/- 3 mV (n = 3), a value close to Erev for Na(+)-permeable ion channels present in oocytes. These same currents were reduced significantly when extracellular Na+ was substituted with choline and Erev changed to -30 +/- 5 mV (n = 3). Inward currents evoked by ATP and UTP were inhibited in a dose-dependent manner by amiloride (10-100 microM). 5. Fast and slow inward currents caused by 2MeSATP reversed at -29 +/- 3 mV (n = 3), a value close to Erev for Cl- channels in oocytes. Fast and slow responses to 2MeSATP were inhibited in a dose-dependent manner by the Cl- channel blocker NPPB (10-100 microM) and were only reduced slightly by amiloride (100 microM). 6. We conclude that two P2 purinoceptor subtypes are present on follicular Xenopus oocytes. 2MeSATP activates fast and slow Cl- currents (Erev = -30 mV) via a suramin-insensitive P2Y purinoceptor, while UTP and ATP activate fast and slow Na(+)-dependent currents (Erev = -10 mV) via a suramin-sensitive P2U purinoceptor.
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