ArticleNucleic acids research1996
UGA suppression by tRNACmCATrp occurs in diverse virus RNAs due to a limited influence of the codon context.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Factors Affecting Readthrough of Natural Versus Premature Termination Codons.Advances in experimental medicine and biology · 2023Review
- Tissue-specific regulation of translational readthrough tunes functions of the traffic jam transcription factor.Nucleic acids research · 2022Article
- 2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations.Nature communications · 2020Article
- Translational recoding: canonical translation mechanisms reinterpreted.Nucleic acids research · 2020Review
- Atypical RNA Elements Modulate Translational Readthrough in Tobacco Necrosis Virus D.Journal of virology · 2017Article
- Changes in protein domains outside the catalytic site of the bacteriophage Qβ replicase reduce the mutagenic effect of 5-azacytidine.Journal of virology · 2014Article
- Known and novel post-transcriptional regulatory sequences are conserved across plant families.RNA (New York, N.Y.) · 2012Article
- Characterization of the stop codon readthrough signal of Colorado tick fever virus segment 9 RNA.RNA (New York, N.Y.) · 2012Article
- Post-transcriptionally regulated expression system in human xenogeneic transplantation models.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Stimulation of stop codon readthrough: frequent presence of an extended 3' RNA structural element.Nucleic acids research · 2011Article
- Versatile dual reporter gene systems for investigating stop codon readthrough in plants.PloS one · 2009Article
- Control of gag-pol gene expression in the Candida albicans retrotransposon Tca2.BMC molecular biology · 2007Article
- Predominance of six different hexanucleotide recoding signals 3' of read-through stop codons.Nucleic acids research · 2002Article
- Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.Nucleic acids research · 2001Review
- Nucleotide sequences and functional characterization of two tobacco UAG suppressor tRNA(Gln) isoacceptors and their genes.Plant molecular biology · 1998Article
- Wheat cytoplasmic arginine tRNA isoacceptor with a U*CG anticodon is an efficient UGA suppressor in vitro.Nucleic acids research · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently identified chloroplast and cytoplasmic tRNACmCATrp as the first natural UGA suppressor tRNAs in plants. The interaction of these tRNAs with UGA involves a Cm: A mismatch at the first anticodon position. We show here that tRNACmCATrp is incapable of misreading UAA and UAG codons in vitro, implying that unconventional base pairs are not tolerated in the middle anticodon position. Furthermore, we demonstrate that the ability of tRNACmCATrp to promote UGA read-through depends on a quite simple codon context. Part of the sequence surrounding the leaky UGA stop codon in tobacco rattle virus RNA-1 was subcloned into a zein reporter gene and read-through efficiency was measured by translation of RNA transcripts in wheat germ extract. A number of mutations in the codons adjacent to the UGA were introduced by site-directed mutagenesis. It was found that single nucleotide exchanges at either side of the UGA had little effect on read-through efficiency. A pronounced influence on suppression by tRNACmCATrp was seen only if 2 or 3 nt at the 3'-side of the UGA codon had been simultaneously replaced. As a consequence of the flexible codon context accepted by tRNACmCATrp, this tRNA is able to misread the UGA in a number of plant and animal viral RNAs that use translational read-through for expression of some of their genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.