ArticleNucleic acids research1996
The reverse two-hybrid system: a genetic scheme for selection against specific protein/protein interactions.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
- Mechanisms and Research Methods of Protein Modification in Virus Entry.Applied biochemistry and biotechnology · 2025Review
- A novel reverse two-hybrid method for the identification of missense mutations that disrupt protein-protein binding.Scientific reports · 2020Article
- Methodologies for Backbone Macrocyclic Peptide Synthesis Compatible With Screening Technologies.Frontiers in chemistry · 2020Review
- Review
- Article
- Techniques for the Analysis of Protein-Protein Interactions in Vivo.Plant physiology · 2016Review
- Review
- High-resolution network biology: connecting sequence with function.Nature reviews. Genetics · 2013Review
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- 'Edgetic' perturbation of a C. elegans BCL2 ortholog.Nature methods · 2009Article
- Residues clustered in the light-sensing knot of phytochrome B are necessary for conformer-specific binding to signaling partner PIF3.PLoS genetics · 2009Article
- The geminivirus nuclear shuttle protein NSP inhibits the activity of AtNSI, a vascular-expressed Arabidopsis acetyltransferase regulated with the sink-to-source transition.Plant physiology · 2006Article
- A systematic method for identifying small-molecule modulators of protein-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Interaction of the movement protein NSP and the Arabidopsis acetyltransferase AtNSI is necessary for Cabbage leaf curl geminivirus infection and pathogenicity.Journal of virology · 2004Article
- Analysing protein-protein interactions with the yeast two-hybrid system.Plant molecular biology · 2002Review
- Heterologous dimerization domains functionally substitute for the double-stranded RNA binding domains of the kinase PKR.The EMBO journal · 2001Article
- The yeast two-hybrid system and its pharmaceutical significance.Pharmaceutical research · 2000Review
- Yeast Two-Hybrid: State of the Art.Biological procedures online · 1999Article
- An artificial cell-cycle inhibitor isolated from a combinatorial library.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The yeast two-hybrid system is a powerful experimental approach for the characterization of protein/ protein interactions. A unique strength of the yeast two-hybrid system is the provision for genetic selection techniques that enable the identification of specific protein/protein interactions. We now report the development of a modified yeast two-hybrid system which enables genetic selection against a specific protein/protein interaction. This reverse two-hybrid system utilizes a yeast strain which is resistant to cycloheximide due to the presence of a mutant cyh2 gene. This strain also contains the wild-type CYH2 allele under the transcriptional control of the Gal1 promoter. Expression of the wild-type Gal4 protein is sufficient to restore growth sensitivity to cycloheximide. Growth sensitivity towards cycloheximide is also restored by the coexpression of the avian c-Rel protein and its I kappa B alpha counterpart, p40, as Gal4 fusion proteins. Restoration of growth sensitivity towards cycloheximide requires the association of c-Rel and p40 at the Gal1 promoter and correlates with the ability of the c-Rel/p40 interaction to activate expression from the Gal1 promoter. A genetic selection scheme against specific protein/protein interactions may be a valuable tool for the analysis of protein/protein interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.