ArticleThe Biochemical journal1996
Release of secretory phospholipase A2 from rat neuronal cells and its possible function in the regulation of catecholamine secretion.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 100 citations in OpenAlex.
- Article
- Translationally Controlled Tumor Protein Stimulates Dopamine Release from PC12 Cells via CaInternational journal of molecular sciences · 2016Article
- NGF induces the expression of group IIA secretory phospholipase A2 in PC12 cells: the newly synthesized enzyme is addressed to growing neurites.Molecular neurobiology · 2014Article
- The role of secretory phospholipase A₂ in the central nervous system and neurological diseases.Molecular neurobiology · 2014Review
- Secreted phospholipase A2-IIA-induced a phenotype of activated microglia in BV-2 cells requires epidermal growth factor receptor transactivation and proHB-EGF shedding.Journal of neuroinflammation · 2012Article
- Role of cytosolic calcium-dependent phospholipase A2 in Alzheimer's disease pathogenesis.Molecular neurobiology · 2012Review
- Role of calcium independent phospholipase A2 in maintaining mitochondrial membrane potential and preventing excessive exocytosis in PC12 cells.Neurochemical research · 2011Article
- Extracellular-derived calcium does not initiate in vivo neurotransmission involving docosahexaenoic acid.Journal of lipid research · 2010Article
- Low molecular weight phospholipases A2 in mammalian brain and neural cells: roles in functions and dysfunctions.Molecular neurobiology · 2010Review
- Differential effects of lysophospholipids on exocytosis in rat PC12 cells.Journal of neural transmission (Vienna, Austria : 1996) · 2010Article
- Bipolar disorder and mechanisms of action of mood stabilizers.Brain research reviews · 2009Review
- Role of secretory phospholipase a(2) in CNS inflammation: implications in traumatic spinal cord injury.CNS & neurological disorders drug targets · 2008Review
- Differential effects of polyunsaturated fatty acids on membrane capacitance and exocytosis in rat pheochromocytoma-12 cells.Neurochemical research · 2006Article
- Lipid signaling in neural plasticity, brain repair, and neuroprotection.Molecular neurobiology · 2005Review
- Inhibitory effects of lysophosphatidylcholine on the dopaminergic system.Neurochemical research · 2004Article
- Secretory phospholipases A2 induce neurite outgrowth in PC12 cells.The Biochemical journal · 2003Article
- Secretory phospholipase A2 activity in the normal and kainate injected rat brain, and inhibition by a peptide derived from python serum.Experimental brain research · 2003Article
- Article
- Chronic lithium treatment decreases brain phospholipase A2 activity.Neurochemical research · 1998Article
- Inhibiting spinal secretory phospholipase AMolecular painArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we show that secretory phospholipase A2 (sPLA2) that is immunochemically indistinguishable from type II sPLA2 is (i) stored in neuroendocrine cells, (ii) released in response to neurotransmitters or depolarization, and (iii) involved in the regulation of catecholamine secretion by these cells. Rat brain synaptic vesicle fractions contained PLA2 activity, which was neutralized completely by an antibody raised against rat type II sPLA2. sPLA2 immunoreactive with anti-(type II sPLA2) antibody was released from synaptosomes in response to depolarization evoked by a high concentration of potassium in the presence of Ca2+. Rat pheochromocytoma PC12 cells, which differentiated into adherent cells similar to sympathetic neurons in response to nerve growth factor, were used for the detailed analysis of the dynamics and function of sPLA2 in neuronal cells. Antibody against rat type II sPLA2 precipitated approximately 80% of the PLA2 activity in PC12 cell lysates. Transcript for type II sPLA2 was detected in PC12 cells by reverse transcriptase-PCR. When neuronally differentiated PC12 cells were stimulated with carbamylcholine or potassium, sPLA2 was released into the medium and reached a maximal approximately 40% release by 15 min. Inhibitors specific to type II sPLA2 suppressed catecholamine secretion by PC12 cells which had been activated by carbamylcholine. Furthermore, treatment of PC12 cells with exogenous type II sPLA2 alone elicited catecholamine secretion. These observations indicate that sPLA2 released from neuronal cells may regulate the degranulation process leading to release of neurotransmitters and are compatible with our earlier finding that this enzyme is involved in the degranulation of rat mast cells.
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