ArticleJournal of bacteriology1996
Absence of light-induced proton extrusion in a cotA-less mutant of Synechocystis sp. strain PCC6803.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- MgPhotosynthesis research · 2024Article
- Hidden Allee effect in photosynthetic organisms.Communicative & integrative biology · 2020Article
- Understanding How Microorganisms Respond to Acid pH Is Central to Their Control and Successful Exploitation.Frontiers in microbiology · 2020Article
- The stress-induced SCP/HLIP family of small light-harvesting-like proteins (ScpABCDE) protects Photosystem II from photoinhibitory damages in the cyanobacterium Synechocystis sp. PCC 6803.Photosynthesis research · 2018Article
- Genetic variations in ARE1 mediate grain yield by modulating nitrogen utilization in rice.Nature communications · 2018Article
- Proton Gradients and Proton-Dependent Transport Processes in the Chloroplast.Frontiers in plant science · 2016Review
- Proteomic analysis reveals resistance mechanism against biofuel hexane in Synechocystis sp. PCC 6803.Biotechnology for biofuels · 2012Article
- Carbon dioxide concentrating mechanism in Chlamydomonas reinhardtii: inorganic carbon transport and CO2 recapture.Photosynthesis research · 2011Review
- alpha-Tocopherol plays a role in photosynthesis and macronutrient homeostasis of the cyanobacterium Synechocystis sp. PCC 6803 that is independent of its antioxidant function.Plant physiology · 2006Article
- PCR analysis of pulsed-field gel electrophoresis-purified plastid DNA, a sensitive tool to judge the hetero-/homoplastomic status of plastid transformants.Current genetics · 2003Article
- Inorganic carbon acquisition systems in cyanobacteria.Photosynthesis research · 2003Article
- Photosynthetic electron transport involved in PxcA-dependent proton extrusion in Synechocystis sp. Strain PCC6803: effect of pxcA inactivation on CO2, HCO3-, and NO3- uptake.Journal of bacteriology · 1998Article
- Disruption of the plastid ycf10 open reading frame affects uptake of inorganic carbon in the chloroplast of Chlamydomonas.The EMBO journal · 1997Article
- Size of cotA and identification of the gene product in Synechocystis sp. strain PCC6803.Journal of bacteriology · 1997Article
- A minute-scale P515 kinetic traces light-driven proton gradient formation in dark-incubated Synechocystis sp. PCC 6803.Physiologia plantarumArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
cotA of Synechocystis sp. strain PCC6803 was isolated as a gene that complemented a mutant defective in CO2 transport and is homologous to cemA that encodes a chloroplast envelope membrane protein (A. Katoh, K.S. Lee, H. Fukuzawa, K. Ohyama, and T. Ogawa, Proc. Natl. Acad. Sci. USA 93:4006-4010, 1996). A mutant (M29) constructed by replacing cotA in the wild-type (WT) Synechocystis strain with the omega fragment was unable to grow in BG11 medium (approximately 17 mM Na+) at pH 6.4 or at any pH in a low-sodium medium (100 microM Na+) under aeration with 3% (vol/vol) CO2 in air. The WT cells grew well in the pH range between 6.4 and 8.5 in BG11 medium but only at alkaline pH in the low-sodium medium. Illumination of the WT cells resulted in an extrusion followed by an uptake of protons. In contrast, only proton uptake was observed for the M29 mutant in the light without proton extrusion. There was no difference in sodium uptake activity between the WT and mutant. The mutant still possessed 51% of the WT CO2 transport activity in the presence of 15 mM NaCl. On the basis of these results we concluded that cotA has a role in light-induced proton extrusion and that the inhibition of CO2 transport in the M29 mutant is a secondary effect of the inhibition of proton extrusion.
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