ArticleJournal of bacteriology1996
The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Assembly, architecture and functional roles of microbial surface layers.Nature reviews. Microbiology · 2026Review
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- Comparison of the Immunogenic Properties ofFrontiers in microbiology · 2022Article
- Functional role of surface layer proteins ofBioscience of microbiota, food and health · 2021Article
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- Constitutive expression and cell-surface display of a bacterial β-mannanase in Lactobacillus plantarum.Microbial cell factories · 2019Article
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- Engineering Components of theFrontiers in microbiology · 2018Review
- Colonization of the Intestinal Tract of the Polyphagous Pest Spodoptera littoralis with the GFP-Tagged Indigenous Gut Bacterium Enterococcus mundtii.Frontiers in microbiology · 2016Article
- Article
- Heterologous Expression of Mannanase and Developing a New Reporter Gene System in Lactobacillus casei and Escherichia coli.PloS one · 2015Article
- Coexpression and secretion of endoglucanase and phytase genes in Lactobacillus reuteri.International journal of molecular sciences · 2014Article
- Lactobacillus surface layer proteins: structure, function and applications.Applied microbiology and biotechnology · 2013Review
- Development of Tools for Genetic Analysis of Phenanthrene Degradation and Nanopod Production by Delftia sp. Cs1-4.Frontiers in microbiology · 2011Article
- Transformation of, and heterologous protein expression in, Lactobacillus agilis and Lactobacillus vaginalis isolates from the chicken gastrointestinal tract.Applied and environmental microbiology · 2011Article
- A novel heme a insertion factor gene cotranscribes with the Thermus thermophilus cytochrome ba3 oxidase locus.Journal of bacteriology · 2010Article
- Comparison of expression vectors in Lactobacillus reuteri strains.FEMS microbiology letters · 2010Article
- Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356.Applied and environmental microbiology · 2008Article
- Extracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactis.Applied and environmental microbiology · 2008Article
- A temperature-sensitive expression system based on the Geobacillus stearothermophilus NRS 2004/3a sgsE surface-layer gene promoter.Biotechnology and applied biochemistry · 2008Article
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5 authors.
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Abstract
S-proteins are proteins which form a regular structure (S-layer) on the outside of the cell walls of many bacteria. Two S-protein-encoding genes are located in opposite directions on a 6.0-kb segment of the chromosome of Lactobacillus acidophilus ATCC 4356 bacteria. Inversion of this chromosomal segment occurs through recombination between two regions with identical sequences, thereby interchanging the expressed and the silent genes. In this study, we show that the region involved in recombination also has a function in efficient S-protein production. Two promoter sequences are present in the S-protein gene expression site, although only the most downstream promoter (P-1) is used to direct mRNA synthesis. S-protein mRNA directed by this promoter has a half-life of 15 min. Its untranslated leader can form a stable secondary structure in which the 5' end is base paired, whereas the ribosome-binding site is exposed. Truncation of this leader sequence results in a reduction in protein production, as shown by reporter gene analysis of Lactobacillus casei. The results obtained indicate that the untranslated leader sequence of S-protein mRNA is involved in efficient S-protein production.
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