ArticleGenetics1996
Bacteriophage T4 mutants hypersensitive to an antitumor agent that induces topoisomerase-DNA cleavage complexes.
Article in Genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Insight into meiotic DNA end resection: Mechanisms and regulation.DNA repair · 2025Review
- Mechanisms and Regulation of DNA-Protein Crosslink Repair During DNA Replication by SPRTN Protease.Frontiers in molecular biosciences · 2022Review
- Excision repair of topoisomerase DNA-protein crosslinks (TOP-DPC).DNA repair · 2020Review
- DNA-protein cross-link repair: what do we know now?Cell & bioscience · 2020Review
- DNA- and DNA-Protein-Crosslink Repair in Plants.International journal of molecular sciences · 2019Review
- Mechanisms of DNA-protein crosslink repair.Nature reviews. Molecular cell biology · 2017Review
- Autoinhibition of bacteriophage T4 Mre11 by its C-terminal domain.The Journal of biological chemistry · 2014Article
- Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives.Virology journal · 2010Review
- Regression supports two mechanisms of fork processing in phage T4.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Yeast recombination pathways triggered by topoisomerase II-mediated DNA breaks.Nucleic acids research · 2003Article
- Bacteriophage T4 genome.Microbiology and molecular biology reviews : MMBR · 2003Review
- The tight linkage between DNA replication and double-strand break repair in bacteriophage T4.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- UvsW protein regulates bacteriophage T4 origin-dependent replication by unwinding R-loops.Molecular and cellular biology · 2001Article
- Article
- Intron homing with limited exon homology. Illegitimate double-strand-break repair in intron acquisition by phage t4.Genetics · 1999Article
- Bacteriophage T4 rnh (RNase H) null mutations: effects on spontaneous mutation and epistatic interaction with rII mutations.Journal of bacteriology · 1999Article
- The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Article
- Bacteriophage T4 UvsW protein is a helicase involved in recombination, repair and the regulation of DNA replication origins.The EMBO journal · 1997Article
- Either bacteriophage T4 RNase H or Escherichia coli DNA polymerase I is essential for phage replication.Journal of bacteriology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Many antitumor agents and antibiotics affect cells by interacting with type II topoisomerases, stabilizing a covalent enzyme-DNA complex. A pathway of recombination can apparently repair this DNA damage. In this study, transposon mutagenesis was used to identify possible components of the repair pathway in bacteriophage T4. Substantial increases in sensitivity to the antitumor agent m-AMSA [4'-(9-acridinylamino)methanesulfon-m-anisidide] were found with transposon insertion mutations that inactivate any of six T4-encoded proteins: UvsY (DNA synaptase accessory protein), UvsW (unknown function), Rnh (RNase H and 5' to 3' DNA exonuclease), alpha-gt (alpha-glucosyl transferase), gp47.1 (uncharacterized), and NrdB (beta subunit of ribonucleotide reductase). The role of the rnh gene in drug sensitivity was further characterized. First, an in-frame rnh deletion mutation was constructed and analyzed, providing evidence that the absence of Rnh protein causes hypersensitivity to m-AMSA. Second, the m-AMSA sensitivity of the rnh-deletion mutant was shown to require a drug-sensitive T4 topoisomerase. Third, analysis of double mutants suggested that uvsW and rnh mutations impair a common step in the recombinational repair pathway for m-AMSA-induced damage. Finally, the rnh-deletion mutant was found to be hypersensitive to UV, implicating Rnh in recombinational repair of UV-induced damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.