ArticlePlant molecular biology1996
Differential regulation of small heat-shock genes in plants: analysis of a water-stress-inducible and developmentally activated sunflower promoter.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 92 citations in OpenAlex.
- Comparing four heat-inducible promoters in stably transformed sugarcane regarding spatial and temporal control of transgene expression reveals candidates to drive stem-preferred transgene expression.Frontiers in plant science · 2025Article
- Expression analysis of nine small heat shock protein genes from Tamarix hispida in response to different abiotic stresses and abscisic acid treatment.Molecular biology reports · 2014Article
- Temporal and spatial control of gene expression in horticultural crops.Horticulture research · 2014Review
- MicroRNA-mediated gene regulation: potential applications for plant genetic engineering.Plant molecular biology · 2013Review
- Isolation of the Chinese rose sHSP gene promoter and its differential regulation analysis in transgenic Arabidopsis plants.Molecular biology reports · 2012Article
- Analysis of the Wsi18, a stress-inducible promoter that is active in the whole grain of transgenic rice.Transgenic research · 2011Article
- The molecular basis for stress-induced acquisition of somatic embryogenesis.Molecular biology reports · 2010Review
- Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana.BMC plant biology · 2008Article
- The plant sHSP superfamily: five new members in Arabidopsis thaliana with unexpected properties.Cell stress & chaperones · 2008Article
- Distinct heat-shock element arrangements that mediate the heat shock, but not the late-embryogenesis induction of small heat-shock proteins, correlate with promoter activation in root-knot nematode feeding cells.Plant molecular biology · 2008Article
- Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways.BMC genomics · 2007Article
- A novel transcriptional cascade regulating expression of heat stress proteins during seed development of Arabidopsis.The Plant cell · 2007Article
- Hahb-4, a sunflower homeobox-leucine zipper gene, is a developmental regulator and confers drought tolerance to Arabidopsis thaliana plants.Transgenic research · 2005Article
- Small heat shock proteins are differentially regulated during pollen development and following heat stress in tobacco.Plant molecular biology · 2005Article
- Comparative transcriptional profiling of placenta and endosperm in developing maize kernels in response to water deficit.Plant physiology · 2003Article
- Selective activation of the developmentally regulated Ha hsp17.6 G1 promoter by heat stress transcription factors.Plant physiology · 2002Article
- Reversible heat-induced inactivation of chimeric beta-glucuronidase in transgenic plants.Plant physiology · 2002Article
- Differential gene expression during somatic embryogenesis in Coffea arabica L., revealed by RT-PCR differential display.Molecular biotechnology · 2002Article
- Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana.Cell stress & chaperones · 2001Article
- The expression of small heat shock proteins in seeds responds to discrete developmental signals and suggests a general protective role in desiccation tolerance.Plant physiology · 2000Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated two sunflower genes, Ha hsp18.6 G2 and Ha hsp17.7 G4, that encode small heat shock proteins (sHSPs). RNAse A protection experiments, carried out with RNA probes transcribed from each gene and hybridized to sunflower total RNA, allowed us to distinguish their mRNA accumulation patterns. In sunflower, Ha hsp17.7 G4 mRNAs accumulated during zygotic embryogenesis at 25 degrees C. In vegetative tissues, these mRNAs accumulated in response to either heat shock (42 degrees C), abscisic acid (ABA), or mild water stress treatments. In all cases, the mRNAs were transcribed from the same initiation site. In contrast, Ha hsp 18.6 G2 mRNAs accumulated only in response to heat-shock. This result demonstrates differential regulation of these two sHSP genes. The complex regulation depicted by the Ha hsp 17.7 G4 promoter has been further analyzed in transgenic tobacco, using G4::GUS translational fusions. Developmental induction of Ha hsp 17.7 G4 during zygotic embryogenesis was faithfully reproduced in the transgenic plants. 5'-distal sequences (between -1132 and -395) were required to confer a preferential spatial expression of GUS activity in the cotyledons. More proximal sequences (from -83 to +163) conferred to the chimeric genes most of the developmental regulation, and the responses to ABA and heat shock characteristic of the Ha hsp17.7 G4 promoter. The water stress response of this gene was not reproduced in transgenic tobacco and, thus, could be uncoupled from its regulation during embryogenesis.
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