ArticleBiophysical journal1996
Scanning two-photon fluctuation correlation spectroscopy: particle counting measurements for detection of molecular aggregation.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 149 citations in OpenAlex.
- Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cationic Polyelectrolyte Adsorption onto Anionic Nanoparticles Analyzed with Frequency-Domain Scanning Fluorescence Correlation Spectroscopy.Small methods · 2025Article
- Pair correlation microscopy of intracellular molecular transport.Nature protocols · 2025Review
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Using FCS to accurately measure protein concentration in the presence of noise and photobleaching.Biophysical journal · 2021Article
- Development and application of multicolor burst analysis spectroscopy.Biophysical journal · 2021Article
- VenusBiophysical journal · 2019Article
- Arbitrary-Region Raster Image Correlation Spectroscopy.Biophysical journal · 2016Article
- Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness.Nature communications · 2016Article
- Article
- From fast fluorescence imaging to molecular diffusion law on live cell membranes in a commercial microscope.Journal of visualized experiments : JoVE · 2014Article
- Quantifying intracellular protein binding thermodynamics during mechanotransduction based on FRET spectroscopy.Methods (San Diego, Calif.) · 2014Article
- Imaging methodologies for systems biology.Cell adhesion & migration · 2014Review
- Enhanced emission of fluorophores on shrink-induced wrinkled composite structures.Optical materials express · 2014Article
- τFCS: multi-method global analysis enhances resolution and sensitivity in fluorescence fluctuation measurements.PloS one · 2014Article
- Fast spatiotemporal correlation spectroscopy to determine protein lateral diffusion laws in live cell membranes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Dual channel detection of ultra low concentration of bacteria in real time by scanning FCS.Measurement science & technology · 2013Article
- Measurement of distance with the nanoscale precise imaging by rapid beam oscillation method.Microscopy research and technique · 2012Article
- Fluorescence correlation spectroscopy study of protein transport and dynamic interactions with clustered-charge peptide adsorbents.Journal of molecular recognition : JMR · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Scanning fluctuation correlation spectroscopy (FCS) is an experimental technique capable of measuring particle number concentrations by monitoring spontaneous equilibrium fluctuations in the local concentration of a fluorescent species in a small (femtoliter) subvolume of a sample. The method can be used to detect molecular aggregation for dilute, submicromolar samples by directly "counting particles". We introduce the application of two-photon excitation to scanning FCS and discuss its important advantages for this technique. We demonstrate the capability of measuring particle number concentrations in solution, first with dilute samples of monodisperse 7-nm and 15-nm radius latex spheres, and then with B phycoerythrin. The detection of multiple species in a single sample is shown, using mixtures containing both sphere sizes. The method is then applied to study protein aggregation in solution. We monitor the concentration-dependent association/ dissociation equilibrium for glycogen phosphorylase A and malate dehydrogenase. The measured dissociation constants, 430 nM and 144 nM respectively, are in good agreement with previously published values. In addition, oligomer dissociation induced by pH titration from pH 8 to pH 5.0 is detectable for the enyme phosphofructokinase. The possibility of measuring dissociation kinetics by scanning two-photon FCS is also demonstrated using phosphofructokinase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.