ArticleBiophysical journal1996
Surface plasmon resonance spectroscopy studies of membrane proteins: transducin binding and activation by rhodopsin monitored in thin membrane films.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 82 citations in OpenAlex.
- Kinetics of cone specific G-protein signaling in avian photoreceptor cells.Frontiers in molecular neuroscience · 2023Article
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- Plasmon Waveguide Resonance: Principles, Applications and Historical Perspectives on Instrument Development.Molecules (Basel, Switzerland) · 2021Review
- Development of a Novel SPR Assay to Study CXCR4-Ligand Interactions.Biosensors · 2020Article
- Surface plasmon resonance applied to G protein-coupled receptors.Biomedical spectroscopy and imaging · 2013Article
- The cytoplasmic rhodopsin-protein interface: potential for drug discovery.Current drug targets · 2012Review
- Role of membrane integrity on G protein-coupled receptors: Rhodopsin stability and function.Progress in lipid research · 2011Review
- Contribution of membrane elastic energy to rhodopsin function.Biophysical journal · 2010Article
- Complexes between photoactivated rhodopsin and transducin: progress and questions.The Biochemical journal · 2010Review
- Surface plasmon resonance for high-throughput ligand screening of membrane-bound proteins.Biotechnology journal · 2009Review
- Membrane functional organisation and dynamic of mu-opioid receptors.Cellular and molecular life sciences : CMLS · 2009Review
- Isolation and functional characterization of a stable complex between photoactivated rhodopsin and the G protein, transducin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Lipid-rhodopsin hydrophobic mismatch alters rhodopsin helical content.Journal of the American Chemical Society · 2008Article
- Curvature and hydrophobic forces drive oligomerization and modulate activity of rhodopsin in membranes.Biophysical journal · 2006Article
- Plasmon resonance methods in GPCR signaling and other membrane events.Current protein & peptide science · 2005Review
- Phosphatidylethanolamine enhances rhodopsin photoactivation and transducin binding in a solid supported lipid bilayer as determined using plasmon-waveguide resonance spectroscopy.Biophysical journal · 2005Article
- Plasmon-waveguide resonance and impedance spectroscopy studies of the interaction between penetratin and supported lipid bilayer membranes.Biophysical journal · 2003Article
- Independent and synergistic interaction of retinal G-protein subunits with bovine rhodopsin measured by surface plasmon resonance.The Biochemical journal · 2001Article
- Optical anisotropy in lipid bilayer membranes: coupled plasmon-waveguide resonance measurements of molecular orientation, polarizability, and shape.Biophysical journal · 2001Article
- Plasmon resonance studies of agonist/antagonist binding to the human delta-opioid receptor: new structural insights into receptor-ligand interactions.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Surface plasmon resonance (SPR) spectroscopy can provide useful information regarding average structural properties of membrane films supported on planar solid substrates. Here we have used SPR spectroscopy for the first time to monitor the binding and activation of G-protein (transducin or Gt) by bovine rhodopsin incorporated into an egg phosphatidylcholine bilayer deposited on a silver film. Rhodopsin incorporation into the membrane, performed by dilution of a detergent solution of the protein, proceeds in a saturable manner. Before photolysis, the SPR data show that Gt binds tightly (Keq approximately equal to 60 nM) and with positive cooperativity to rhodopsin in the lipid layer to form a closely packed film. A simple multilayer model yields a calculated average thickness of about 57 A, in good agreement with the structure of Gt. The data also demonstrate that Gt binding saturates at a Gt/rhodopsin ratio of approximately 0.6. Moreover, upon visible light irradiation, characteristic changes occur in the SPR spectrum, which can be modeled by a 6 A increase in the average thickness of the lipid/protein film caused by formation of metarhodopsin II (MII). Upon subsequent addition of GTP, further SPR spectral changes are induced. These are interpreted as resulting from dissociation of the alpha-subunit of Gt, formation of new MII-Gt complexes, and possible conformational changes of Gt as a consequence of complex formation. The above results clearly demonstrate the ability of SPR spectroscopy to monitor interactions among the proteins associated with signal transduction in membrane-bound systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.