ArticleBiophysical journal1996
A Ba2+ chelator suppresses long shut events in fully activated high-conductance Ca(2+)-dependent K+ channels.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Mutation in pore-helix modulates interplay between filter gate and Ba2+ block in a Kcv channel pore.The Journal of general physiology · 2024Article
- Mg²⁺ modulation of the single-channel properties of KCa3.1 in human erythroleukemia cells.Pflugers Archiv : European journal of physiology · 2014Article
- Closed-channel block of BK potassium channels by bbTBA requires partial activation.The Journal of general physiology · 2009Article
- An extracellular Cu2+ binding site in the voltage sensor of BK and Shaker potassium channels.The Journal of general physiology · 2008Article
- Mg2+ enhances voltage sensor/gate coupling in BK channels.The Journal of general physiology · 2008Article
- State-dependent block of BK channels by synthesized shaker ball peptides.The Journal of general physiology · 2006Article
- Role of charged residues in the S1-S4 voltage sensor of BK channels.The Journal of general physiology · 2006Article
- Intracellular Mg2+ influences both open and closed times of a native Ca2+-activated BK channel in cultured human renal proximal tubule cells.The Journal of membrane biology · 2005Article
- Cysteine modification alters voltage- and Ca(2+)-dependent gating of large conductance (BK) potassium channels.The Journal of general physiology · 2005Article
- Unique inner pore properties of BK channels revealed by quaternary ammonium block.The Journal of general physiology · 2004Article
- Effect of phosphatidylserine on unitary conductance and Ba2+ block of the BK Ca2+-activated K+ channel: re-examination of the surface charge hypothesis.The Journal of general physiology · 2003Article
- Coupling between voltage sensor activation, Ca2+ binding and channel opening in large conductance (BK) potassium channels.The Journal of general physiology · 2002Article
- Complex voltage-dependent behavior of single unliganded calcium-sensitive potassium channels.Biophysical journal · 2000Article
- Localization of the K+ lock-In and the Ba2+ binding sites in a voltage-gated calcium-modulated channel. Implications for survival of K+ permeability.The Journal of general physiology · 1999Article
- Allosteric voltage gating of potassium channels I. Mslo ionic currents in the absence of Ca(2+).The Journal of general physiology · 1999Article
- Allosteric voltage gating of potassium channels II. Mslo channel gating charge movement in the absence of Ca(2+).The Journal of general physiology · 1999Article
- Mode switching characterizes the activity of large conductance potassium channels recorded from rat cortical fused nerve terminals.The Journal of physiology · 1998Article
- Ca2+-dependent inactivation of large conductance Ca2+-activated K+ (BK) channels in rat hippocampal neurones produced by pore block from an associated particle.The Journal of physiology · 1998Article
- Activation and two modes of blockade by strontium of Ca2+-activated K+ channels in goldfish saccular hair cells.The Journal of general physiology · 1998Article
- Intrinsic voltage dependence and Ca2+ regulation of mslo large conductance Ca-activated K+ channels.The Journal of general physiology · 1997Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-conductance Ca(2+)-activated K+ channels from rat skeletal muscle were incorporated into planar lipid bilayers, and the channel kinetics were studied with a high internal Ca2+ concentration (Cai). Raising the Cai is known to increase the channel open probability. This effect is due to an increases in openings frequency and duration, and saturates at a Cai around 100 microM. Raising the Cai also increases the occurrence of less frequent but very long (> 5 s) shut events. The mechanism underlying this slow kinetic process was studied. Raising Cai above 100 microM does not further increase the frequency of the long shut events. This was not consistent with the hypothesis that the long closures result from a classical channel-block mechanism induced by internal Ca2+. The transmembrane voltage and the presence of K+ ions in the external compartment both affect the slow kinetic process. A comparison of these effects with the properties of the channel block induced by Ba2+ ions added to the internal compartment strongly suggested that the long shut events are due to a contamination of the internal solutions by Ba2+. This was confirmed by showing that a crown-ether compound that strongly chelates Ba2+ completely suppresses the long shut events when added to the inner compartment.
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