ArticleBiophysical journal1996
The orientation of eosin-5-maleimide on human erythrocyte band 3 measured by fluorescence polarization microscopy.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
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- Red blood cells in biology and translational medicine: natural vehicle inspires new biomedical applications.Theranostics · 2024Review
- Oxidized Low-Density Lipoprotein Accumulation Suppresses Glycolysis and Attenuates the Macrophage Inflammatory Response by Diverting Transcription from the HIF-1α to the Nrf2 Pathway.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Apollo-NADPScience advances · 2023Article
- Targeting Apollo-NADPACS sensors · 2022Article
- Cell physiology and molecular mechanism of anion transport by erythrocyte band 3/AE1.American journal of physiology. Cell physiology · 2021Review
- Single molecule optical measurements of orientation and rotations of biological macromolecules.Methods and applications in fluorescence · 2016Article
- Apollo-NADP(+): a spectrally tunable family of genetically encoded sensors for NADP(+).Nature methods · 2016Article
- Role of band 3 in the erythrocyte membrane structural changes under thermal fluctuations -multi scale modeling considerations.Journal of bioenergetics and biomembranes · 2015Review
- Fluorescence linear dichroism imaging for quantifying membrane order.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Probing the orientational distribution of dyes in membranes through multiphoton microscopy.Biophysical journal · 2012Article
- In vivo imaging of the actin polymerization state with two-photon fluorescence anisotropy.Biophysical journal · 2012Article
- Analysis of the mobilities of band 3 populations associated with ankyrin protein and junctional complexes in intact murine erythrocytes.The Journal of biological chemistry · 2012Article
- Probing orientational behavior of MHC class I protein and lipid probes in cell membranes by fluorescence polarization-resolved imaging.Biophysical journal · 2011Article
- Fluorescence anisotropy of protein complexes in living cells.Biophysical journal · 2010Article
- Fluorescence polarization/anisotropy in diagnostics and imaging.Chemical reviews · 2010Review
- Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions.European biophysics journal : EBJ · 2010Article
- Changes in Band 3 oligomeric state precede cell membrane phospholipid loss during blood bank storage of red blood cells.Transfusion · 2009Article
- Probing membrane order and topography in supported lipid bilayers by combined polarized total internal reflection fluorescence-atomic force microscopy.Biophysical journal · 2009Article
- Determination of the orientational distribution and orientation factor for transfer between membrane-bound fluorophores using a confocal microscope.Biophysical journal · 2006Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The dominant motional mode for membrane proteins is uniaxial rotational diffusion about the membrane normal axis, and investigations of their rotational dynamics can yield insight into both the oligomeric state of the protein and its interactions with other proteins such as the cytoskeleton. However, results from the spectroscopic methods used to study these dynamics are dependent on the orientation of the probe relative to the axis of motion. We have employed polarized fluorescence confocal microscopy to measure the orientation of eosin-5-maleimide covalently reacted with Lys-430 of human erythrocyte band 3. Steady-state polarized fluorescence images showed distinct intensity patterns, which were fit to an orientation distribution of the eosin absorption and emission dipoles relative to the membrane normal axis. This orientation was found to be unchanged by trypsin treatment, which cleaves band 3 between the integral membrane domain and the cytoskeleton-attached domain. this result suggests that phosphorescence anisotropy changes observed after trypsin treatment are due to a rotational constraint change rather than a reorientation of eosin. By coupling time-resolved prompt fluorescence anisotropy with confocal microscopy, we calculated the expected amplitudes of the e-Dt and e-4Dt terms from the uniaxial rotational diffusion model and found that the e-4Dt term should dominate the anisotropy decay. Delayed fluorescence and phosphorescence anisotropy decays of control and trypsin-treated band 3 in ghosts, analyzed as multiple uniaxially rotating populations using the amplitudes predicted by confocal microscopy, were consistent with three motional species with uniaxial correlation times ranging from 7 microseconds to 1.4 ms.
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