ArticleBiophysical journal1996
Effects of buffer concentration on voltage-gated H+ currents: does diffusion limit the conductance?
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed.
- Biophysical Properties of Somatic Cancer Mutations in the S4 Transmembrane Segment of the Human Voltage-Gated Proton Channel hHBiomolecules · 2025Article
- Transcendent Aspects of Proton Channels.Annual review of physiology · 2024Review
- Voltage-Gated Proton Channels in the Tree of Life.Biomolecules · 2023Review
- Structural motifs for subtype-specific pH-sensitive gating of vertebrate otopetrin proton channels.eLife · 2022Article
- Expression of HProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Analysis of an electrostatic mechanism for ΔpH dependent gating of the voltage-gated proton channel, HBiochimica et biophysica acta. Bioenergetics · 2021Review
- Zinc modulation of proton currents in a new voltage-gated proton channel suggests a mechanism of inhibition.The FEBS journal · 2020Article
- Zn2+ to probe voltage-gated proton (Hv1) channels.The Journal of general physiology · 2020Article
- Engineered high-affinity zinc binding site reveals gating configurations of a human proton channel.The Journal of general physiology · 2020Article
- Voltage and pH sensing by the voltage-gated proton channel, HJournal of the Royal Society, Interface · 2018Review
- CrossTalk proposal: Proton permeation through HThe Journal of physiology · 2017Article
- The intimate and controversial relationship between voltage-gated proton channels and the phagocyte NADPH oxidase.Immunological reviews · 2016Review
- Insights into the structure and function of HV1 from a meta-analysis of mutation studies.The Journal of general physiology · 2016Review
- Currents through Hv1 channels deplete protons in their vicinity.The Journal of general physiology · 2016Article
- Philosophy of voltage-gated proton channels.Journal of the Royal Society, Interface · 2014Review
- Analysis of electrophysiological properties and responses of neutrophils.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Voltage-gated proton channels: molecular biology, physiology, and pathophysiology of the H(V) family.Physiological reviews · 2013Review
- Consequences of dimerization of the voltage-gated proton channel.Progress in molecular biology and translational science · 2013Review
- Voltage-gated proton channels.Comprehensive Physiology · 2012Review
- A voltage-gated H+ channel underlying pH homeostasis in calcifying coccolithophores.PLoS biology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The single-channel proton conductance of the voltage-gated H(+)-selective channel, like that of the F0 component of the H(+)-ATPase, is nearly constant over a wide range of pH encompassing the physiological range. To examine the possible contributions of buffer diffusion and buffer-channel proton transfer reactions to this phenomenon, the effects of buffer concentration on voltage-activated H+ currents were explored in voltage-clamped rat alveolar epithelial cells. Changes in the external buffer concentration ([B]o), evaluated using the whole-cell configuration, had only small effects on H+ currents (IH). Lowering [B]o from 100 to 1 mM did not alter the voltage-activation curve or reversal potential (Vrev) but reduced IH, typically by 10-30%. Changes in internal buffer concentration ([B]i), examined in inside-out patches, usually altered IH more distinctly and subtly changed the kinetics. Overall, the effects of changing buffer concentration were small and subtle. The maximum attenuation of the single-channel H+ current at 1 mM buffer was estimated to be approximately 20% at either mouth of the H+ channel. Therefore, the rate-determining step in H+ permeation is neither deprotonation of buffer at the inner mouth of the channel nor protonation of buffer at the external surface. Evidently the rate of H+ permeation through the channel is itself small enough that diffusion of buffer in bulk solution does not directly limit the conductance significantly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.