ArticleThe Journal of physiology1996
Endocytosis of secretory granules in mouse pancreatic beta-cells evoked by transient elevation of cytosolic calcium.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 88 citations in OpenAlex.
- SNAP-23 and syntaxin-2 localize to the extracellular surface of the platelet plasma membrane.Blood · 2007Trial
- The highly expressed calcium-insensitive synaptotagmin-11 and synaptotagmin-13 modulate insulin secretion.Acta physiologica (Oxford, England) · 2022Article
- Review
- Dynamin deficiency causes insulin secretion failure and hyperglycemia.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mathematical modeling of white adipocyte exocytosis predicts adiponectin secretion and quantifies the rates of vesicle exo- and endocytosis.The Journal of biological chemistry · 2017Article
- Ultrasound Stimulation of Insulin Release from Pancreatic Beta Cells as a Potential Novel Treatment for Type 2 Diabetes.Ultrasound in medicine & biology · 2017Article
- Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis.The Journal of clinical investigation · 2015Article
- Ultrasound-mediated oncolytic virus delivery and uptake for increased therapeutic efficacy: state of art.Oncolytic virotherapy · 2015Review
- PKA-independent cAMP stimulation of white adipocyte exocytosis and adipokine secretion: modulations by Ca2+ and ATP.The Journal of physiology · 2014Article
- Ultrasound enhanced PEI-mediated gene delivery through increasing the intracellular calcium level and PKC-δ protein expression.Pharmaceutical research · 2014Article
- Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells.Protein & cell · 2012Article
- Evolving insights regarding mechanisms for the inhibition of insulin release by norepinephrine and heterotrimeric G proteins.American journal of physiology. Cell physiology · 2012Review
- On depolarization-evoked exocytosis as a function of calcium entry: possibilities and pitfalls.Biophysical journal · 2011Article
- Effects of shear stress cultivation on cell membrane disruption and intracellular calcium concentration in sonoporation of endothelial cells.Journal of biomechanics · 2011Article
- Hormonal inhibition of endocytosis: novel roles for noradrenaline and G protein G(z).The Journal of physiology · 2010Article
- Quantal ATP release in rat beta-cells by exocytosis of insulin-containing LDCVs.Pflugers Archiv : European journal of physiology · 2009Article
- Insulin granule biogenesis, trafficking and exocytosis.Vitamins and hormones · 2009Review
- GTPase cycle of dynamin is coupled to membrane squeeze and release, leading to spontaneous fission.Cell · 2008Article
- Corelease and differential exit via the fusion pore of GABA, serotonin, and ATP from LDCV in rat pancreatic beta cells.The Journal of general physiology · 2007Article
- Time-dependent mechanisms in beta-cell glucose sensing.Journal of biological physics · 2006Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
1. To investigate the mechanisms regulating the reuptake of secretory granule membranes following regulated exocytosis, we have monitored changes in cell capacitance in single pancreatic beta-cells. 2. Membrane retrieval (endocytosis) occurred both in a continuous manner and in abrupt steps, corresponding to the simultaneous retrieval of 50-100 granules. The large endocytotic steps were associated with a conductance change of about 1 nS which we attribute to the formation of a fission pore with a pore radius of approximately 1 nm. 3. In some cells, we observed large amplitude capacitance fluctuations, suggesting that aggregates of granules are connected to the plasma membrane by a single pore and are subsequently retrieved as a single unit. 4. Endocytosis was evoked by elevation of cytosolic [Ca2+]i, but once initiated, a sustained increase in [Ca2+]i was not required for endocytosis to continue. 5. The [Ca2+]i dependence of exo- and endocytosis was studied by photorelease of Ca2+ from the 'caged' precursor Ca(2+)-nitrophenyl-EGTA (Ca(2+)-NP-EGTA). Both exo- and endocytosis were initiated at between 0.5 and 2 microM Cai(2+). The rate of endocytosis saturated above 2 microM Cai(2+), whereas exocytosis continued to increase up to 4 microM Cai(2+). The maximum rate of endocytosis was < 25% of that of exocytosis. 6. Unlike exocytosis, endocytosis proceeded equally well in the presence or absence of Mg-ATP. 7. Our data indicate that in the pancreatic beta-cell, exocytosis and endocytosis are regulated by different mechanisms.
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