ArticleProceedings of the National Academy of Sciences of the United States of America1996
Phosphorylation by protein kinase C of serine-23 of the alpha-1 subunit of rat Na+,K(+)-ATPase affects its conformational equilibrium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 69 citations in OpenAlex.
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- Identification of a Primary Renal ATCirculation research · 2020Article
- Defective Renal Angiotensin III and ATJournal of the American Heart Association · 2019Article
- Protein kinase C mediates juvenile hormone-dependent phosphorylation of NaThe Journal of biological chemistry · 2018Article
- Evolutionary Analysis of the Lysine-Rich N-terminal Cytoplasmic Domains of the Gastric HThe Journal of membrane biology · 2018Article
- The decrease on Na(+), K(+)-ATPase activity in the cortex, but not in hippocampus, is reverted by antioxidants in an animal model of sepsis.Molecular neurobiology · 2012Article
- Attenuation of neonatal ischemic brain damage using a 20-HETE synthesis inhibitor.Journal of neurochemistry · 2012Article
- Reduced resting potentials in dystrophic (mdx) muscle fibers are secondary to NF-κB-dependent negative modulation of ouabain sensitive Na+-K+ pump activity.Journal of the neurological sciences · 2011Article
- Na+,K+-ATPase is modulated by angiotensin II in diabetic rat kidney--another reason for diabetic nephropathy?The Journal of physiology · 2008Article
- Angiotensin II stimulates elution of Na-K-ATPase from a digoxin-affinity column by increasing the kinetic response to ligands that trigger the decay of E2-P.American journal of physiology. Renal physiology · 2008Article
- RanBP2 modulates Cox11 and hexokinase I activities and haploinsufficiency of RanBP2 causes deficits in glucose metabolism.PLoS genetics · 2006Article
- Mechanisms of Na+-K+-ATPase phosphorylation by PKC in the medullary thick ascending limb of Henle in the rat.Pflugers Archiv : European journal of physiology · 2003Article
- Regulation of sodium/potassium ATPase activity: impact on salt balance and vascular contractility.Current hypertension reports · 2001Review
- Is phosphorylation of the alpha1 subunit at Ser-16 involved in the control of Na,K-ATPase activity by phorbol ester-activated protein kinase C?Molecular biology of the cell · 2000Article
- [Ca2+]i determines the effects of protein kinases A and C on activity of rat renal Na+,K+-ATPase.The Journal of physiology · 1999Article
- Phosphatidylinositol 3-kinase-mediated endocytosis of renal Na+, K+-ATPase alpha subunit in response to dopamine.Molecular biology of the cell · 1998Article
- 20-Hydroxyeicosa-tetraenoic acid (20 HETE) activates protein kinase C. Role in regulation of rat renal Na+,K+-ATPase.The Journal of clinical investigation · 1997Article
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
Abstract
Phosphorylation of the alpha-1 subunit of rat Na+,K(+)-ATPase by protein kinase C has been shown previously to decrease the activity of the enzyme in vitro. We have now undertaken an investigation of the mechanism by which this inhibition occurs. Analysis of the phosphorylation of recombinant glutathione S-transferase fusion proteins containing putative cytoplasmic domains of the protein, site-directed mutagenesis, and two-dimensional peptide mapping indicated that protein kinase C phosphorylated the alpha-1 subunit of the rat Na+,K(+)-ATPase within the extreme NH2-terminal domain, on serine-23. The phosphorylation of this residue resulted in a shift in the equilibrium toward the E1 form, as measured by eosin fluorescence studies, and this was associated with a decrease in the apparent K+ affinity of the enzyme, as measured by ATPase activity assays. The rate of transition from E2 to E1 was apparently unaffected by phosphorylation by protein kinase C. These results, together with previous studies that examined the effects of tryptic digestion of Na+,K(+)-ATPase, suggest that the NH2-terminal domain of the alpha-1 subunit, including serine-23, is involved in regulating the activity of the enzyme.
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