ArticleProceedings of the National Academy of Sciences of the United States of America1996
Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy.Molecules (Basel, Switzerland) · 2021Review
- Labeling DNA for single-molecule experiments: methods of labeling internal specific sequences on double-stranded DNA.Nanoscale · 2011Review
- Analyzing the forces binding a restriction endonuclease to DNA using a synthetic nanopore.Nucleic acids research · 2009Article
- Single molecule transcription profiling with AFM.Nanotechnology · 2007Article
- Polynucleotide adsorption to negatively charged surfaces in divalent salt solutions.Biophysical journal · 2006Article
- Site-specific labeling of supercoiled DNA.Nucleic acids research · 2006Article
- Studying the effect of a charged surface on the interaction of bleomycin with DNA using an atomic force microscope.European biophysics journal : EBJ · 2005Article
- Adsorption of DNA to mica mediated by divalent counterions: a theoretical and experimental study.Biophysical journal · 2003Article
- Mechanics and imaging of single DNA molecules.Journal of muscle research and cell motility · 2002Review
- Atomic force microscopy of the submolecular architecture of hydrated ocular mucins.Biophysical journal · 1999Article
- Scanning force microscopy of DNA molecules elongated by convective fluid flow in an evaporating droplet.Biophysical journal · 1998Article
- Potential role of atomic force microscopy in systems biology.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct imaging with the atomic force microscope has been used to identify specific nucleotide sequences in plasmid DNA molecules. This was accomplished using EcoRI (Gln-111), a mutant of the restriction enzyme that has a 1000-fold greater binding affinity than the wild-type enzyme but with cleavage rate constants reduced by a factor of 10(4). ScaI-linearized plasmids with single (pBS+) and double (pGEM-luc and pSV-beta-galactosidase) EcoRI recognition sites were imaged, and the bound enzyme was localized to a 50- to 100-nt resolution. The high affinity for the EcoRI binding site exhibited by this mutant endonuclease, coupled with an observed low level of nonspecific binding, should prove valuable for physically mapping large DNA clones by direct atomic force microscope imaging.
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