ArticleApplied and environmental microbiology1996
Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- A randomized, blinded, controlled trial investigating the gastrointestinal health effects of drinking water quality.Environmental health perspectives · 2001Trial
- First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern Egypt.BMC research notes · 2025Article
- Assessment of the potential occurrence ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Article
- Detection ofMicroorganisms · 2022Review
- Purification of poly-dA oligonucleotides and mRNA-protein fusions with dTBioorganic & medicinal chemistry letters · 2020Article
- Interaction of Cryptosporidium parvum with mouse dendritic cells leads to their activation and parasite transportation to mesenteric lymph nodes.Pathogens and disease · 2014Article
- The impact of the waterborne transmission of Toxoplasma gondii and analysis efforts for water detection: an overview and update.Environmental science and pollution research international · 2013Review
- Towards a rational strategy for monitoring of microbiological quality of ambient waters.The Science of the total environment · 2012Article
- Detection of viable Cryptosporidium parvum in soil by reverse transcription-real-time PCR targeting hsp70 mRNA.Applied and environmental microbiology · 2011Article
- Development of sensitive detection of cryptosporidium and giardia from surface water in iran.Iranian journal of parasitology · 2011Article
- Stress-induced Hsp70 gene expression and inactivation of Cryptosporidium parvum oocysts by chlorine-based oxidants.Applied and environmental microbiology · 2010Article
- Oligonucleotide-gold nanoparticle networks for detection of Cryptosporidium parvum heat shock protein 70 mRNA.Journal of clinical microbiology · 2009Article
- Pulsed-UV light inactivation of Cryptosporidium parvum.Parasitology research · 2008Article
- CP2 gene as a useful viability marker for Cryptosporidium parvum.Parasitology research · 2008Article
- An evaluation of primers amplifying DNA targets for the detection of Cryptosporidium spp. using C. parvum HNJ-1 Japanese isolate in water samples.Parasitology research · 2007Article
- Quantitative-PCR assessment of Cryptosporidium parvum cell culture infection.Applied and environmental microbiology · 2005Article
- Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors.Applied and environmental microbiology · 2005Article
- Development of a PCR-enzyme immunoassay oligoprobe detection method for Toxoplasma gondii oocysts, incorporating PCR controls.Applied and environmental microbiology · 2003Article
- PCR-restriction fragment length polymorphism analysis of a diagnostic 452-base-pair DNA fragment discriminates between Cryptosporidium parvum and C. meleagridis and between C. parvum isolates of human and animal origin.Applied and environmental microbiology · 2002Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current methods for detection of Cryptosporidium parvum oocysts in water are time-consuming and difficult. We have developed a reverse transcription (RT)-PCR which can detect the presence of a single viable oocyst spiked into concentrated environmental water samples. The test is based on the detection of mRNA from a C. parvum heat shock protein (hsp). The synthesis of hsp was induced by a short 45 degrees C incubation followed by oocyst lysis by a freeze-thaw process. Hsp70 mRNA, produced only from viable oocysts, was then isolated by hybridization to oligo(dT)25-coated magnetic beads. Detection was achieved by RT-PCR amplification of a 590-bp region of hsp70 mRNA specific for C. parvum. To test the method, samples of reticulated, reservoir, bore, and river water were concentrated by chemical flocculation and Percoll-sucrose gradient centrifugation and then spiked with dilutions of oocysts. In all four of the water types examined, the detection of single oocysts was possible by RT-PCR combined with Southern hybridization. RT-PCR products were not obtained from formalin-inactivated oocysts. An RNA internal positive control fragment was synthesized that was included with each reaction to guard against RT-PCR false-negative results that may be caused by the presence of inhibitory substances. However, when the magnetic beads were used to extract and concentrate mRNA, no inhibition was observed. The technique is versatile, straightforward, and rapid (1 day) and provides a sensitive and economic means of screening concentrated water samples for the presence of C. parvum.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.