ArticleApplied and environmental microbiology1996
Activity of the plasma membrane H(+)-ATPase and optimal glycolytic flux are required for rapid adaptation and growth of Saccharomyces cerevisiae in the presence of the weak-acid preservative sorbic acid.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 254 citations in OpenAlex.
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- Perspectives on current and future yeast technologies for ethanol-based biofuels and bioproducts.FEMS yeast research · 2025Review
- Genes controlling hydrolysate toxin tolerance identified by QTL analysis of the natural Saccharomyces cerevisiae BCC39850.Applied microbiology and biotechnology · 2024Article
- Mechanisms and implications of bacterial-fungal competition for soil resources.The ISME journal · 2024Review
- Functional Analysis of the Plasma Membrane HJournal of fungi (Basel, Switzerland) · 2022Article
- The Role of Sch9 and the V-ATPase in the Adaptation Response to Acetic Acid and the Consequences for Growth and Chronological Lifespan.Microorganisms · 2021Article
- Article
- From Acidifiers to Intestinal Health Enhancers: How Organic Acids Can Improve Growth Efficiency of Pigs.Animals : an open access journal from MDPI · 2020Review
- Enhanced lipid production byBiotechnology for biofuels · 2020Article
- Effects of pH and sugar supplements on bacteriocin-like inhibitory substance production by Pediococcus pentosaceus.Molecular biology reports · 2019Article
- Activation of Haa1 and War1 transcription factors by differential binding of weak acid anions in Saccharomyces cerevisiae.Nucleic acids research · 2019Article
- Evolutionary engineering inBiotechnology for biofuels · 2019Article
- Physiological mechanism of improved tolerance ofBiotechnology for biofuels · 2019Article
- Inactivation of the transcription factor mig1 (YGL035C) in Saccharomyces cerevisiae improves tolerance towards monocarboxylic weak acids: acetic, formic and levulinic acid.Journal of industrial microbiology & biotechnology · 2018Article
- Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation.Scientific reports · 2018Article
- Changes in lipid metabolism convey acid tolerance inBiotechnology for biofuels · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The weak acid sorbic acid transiently inhibited the growth of Saccharomyces cerevisiae in media at low pH. During a lag period, the length of which depended on the severity of this weak-acid stress, yeast cells appeared to adapt to this stress, eventually recovering and growing normally. This adaptation to weak-acid stress was not due to metabolism and removal of the sorbic acid. A pma1-205 mutant, with about half the normal membrane H+-ATPase activity, was shown to be more sensitive to sorbic acid than its parent. Sorbic acid appeared to stimulate plasma membrane H+-ATPase activity in both PMA1 and pma1-205. Consistent with this, cellular ATP levels showed drastic reductions, the extent of which depended on the severity of weak-acid stress. The weak acid did not appear to affect the synthesis of ATP because CO2 production and O2 consumption were not affected significantly in PMA1 and pma1-205 cells. However, a glycolytic mutant, with about one-third the normal pyruvate kinase and phosphofructokinase activity and hence a reduced capacity to generate ATP, was more sensitive to sorbic acid than its isogenic parent. These data are consistent with the idea that adaptation by yeast cells to sorbic acid is dependent on (i) the restoration of internal pH via the export of protons by the membrane H+-ATPase in an energy-demanding process and (ii) the generation of sufficient ATP to drive this process and still allow growth.
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