ArticleThe Journal of cell biology1996
Targeting of P-selectin to two regulated secretory organelles in PC12 cells.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migration.PLoS biology · 2019Article
- Quintuple labeling in the electron microscope with genetically encoded enhanced horseradish peroxidase.PloS one · 2018Article
- The redistribution of Drosophila vesicular monoamine transporter mutants from synaptic vesicles to large dense-core vesicles impairs amine-dependent behaviors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Picking faces out of a crowd: genetic labels for identification of proteins in correlated light and electron microscopy imaging.Methods in cell biology · 2012Review
- Article
- Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1.The Journal of cell biology · 2008Article
- Sorting nexin 17 accelerates internalization yet retards degradation of P-selectin.Molecular biology of the cell · 2004Article
- Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor.Molecular biology of the cell · 2002Article
- Sorting to synaptic-like microvesicles from early and late endosomes requires overlapping but not identical targeting signals.Molecular biology of the cell · 2000Article
- A phosphorylation site regulates sorting of the vesicular acetylcholine transporter to dense core vesicles.The Journal of cell biology · 2000Article
- Di-leucine signals mediate targeting of tyrosinase and synaptotagmin to synaptic-like microvesicles within PC12 cells.Molecular biology of the cell · 1999Article
- Secretagogue-triggered transfer of membrane proteins from neuroendocrine secretory granules to synaptic-like microvesicles.Molecular biology of the cell · 1999Article
- A complex web of signal-dependent trafficking underlies the triorganellar distribution of P-selectin in neuroendocrine PC12 cells.The Journal of cell biology · 1999Article
- Role of P-selectin cytoplasmic domain in granular targeting in vivo and in early inflammatory responses.The Journal of cell biology · 1998Article
- Determinants in the cytoplasmic domain of P-selectin required for sorting to secretory granules.The Biochemical journal · 1998Article
- Selectin-carbohydrate interactions during inflammation and metastasis.Glycoconjugate journal · 1997Review
- Routing of membrane proteins to large dense core vesicles in PC12 cells.Journal of molecular neuroscience : MNArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeting of P-selectin to the regulated secretory organelles (RSOs) of phaeochromocytoma PC12 cells has been investigated. By expressing from cDNA a chimera composed of HRP and P-selectin, and then following HRP activity through subcellular fractionation, we have discovered that P-selectin contains signals that target HRP to the synaptic-like microvesicles (SLMV) as well as the dense-core granules (DCGs) of these cells. Mutagenesis of the chimera followed by transient expression in PC12 cells shows that at least two different sequences within the carboxy-terminal cytoplasmic tail of P-selectin are necessary, but that neither is sufficient for trafficking to the SLMV. One of these sequences is centred on the 10 amino acids of the membrane-proximal C1 exon that is also implicated in lysosomal targeting. The other sequence needed for trafficking to the SLMV includes the last four amino acids of the protein. The same series of mutations have a different effect on DCG targeting, showing that traffic to the two different RSOs depends on different features within the cytoplasmic domain of P-selectin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.