ArticleJournal of virology1996
N-butyldeoxynojirimycin-mediated inhibition of human immunodeficiency virus entry correlates with changes in antibody recognition of the V1/V2 region of gp120.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 116 citations in OpenAlex.
- Therapeutic Targeting of Viral N-Glycosylation Modification: From Molecular Mechanisms to Clinical Application Prospects.Infectious diseases and therapy · 2025Review
- Expanding horizons of iminosugars as broad-spectrum anti-virals: mechanism, efficacy and novel developments.Natural products and bioprospecting · 2024Review
- An Amination-Cyclization Cascade Reaction for Iminosugar Synthesis Using Minimal Protecting Groups.ACS omega · 2022Article
- The Role of Glycosylation in Infectious Diseases.Advances in experimental medicine and biology · 2021Review
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- Mechanisms of Antiviral Activity of Iminosugars Against Dengue Virus.Advances in experimental medicine and biology · 2018Review
- Iminosugar antivirals: the therapeutic sweet spot.Biochemical Society transactions · 2017Review
- Article
- Inhibition of endoplasmic reticulum-resident glucosidases impairs severe acute respiratory syndrome coronavirus and human coronavirus NL63 spike protein-mediated entry by altering the glycan processing of angiotensin I-converting enzyme 2.Antimicrobial agents and chemotherapy · 2015Article
- Strain-specific antiviral activity of iminosugars against human influenza A viruses.The Journal of antimicrobial chemotherapy · 2015Article
- Glycosylation and disulfide bond analysis of transiently and stably expressed clade C HIV-1 gp140 trimers in 293T cells identifies disulfide heterogeneity present in both proteins and differences in O-linked glycosylation.Journal of proteome research · 2014Article
- Antiviral therapies targeting host ER alpha-glucosidases: current status and future directions.Antiviral research · 2013Review
- The griffithsin dimer is required for high-potency inhibition of HIV-1: evidence for manipulation of the structure of gp120 as part of the griffithsin dimer mechanism.Antimicrobial agents and chemotherapy · 2013Article
- Variable loop glycan dependency of the broad and potent HIV-1-neutralizing antibodies PG9 and PG16.Journal of virology · 2010Article
- Envelope glycans of immunodeficiency virions are almost entirely oligomannose antigens.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Proximal glycans outside of the epitopes regulate the presentation of HIV-1 envelope gp120 helper epitopes.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- The role of the humoral immune response in the molecular evolution of the envelope C2, V3 and C3 regions in chronically HIV-2 infected patients.Retrovirology · 2008Article
- Review
- The interplay between folding-facilitating mechanisms in Trypanosoma cruzi endoplasmic reticulum.Molecular biology of the cell · 2003Article
- Substrate reduction therapy: clinical evaluation in type 1 Gaucher disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2003Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The alpha-glucosidase inhibitor N-butyldeoxynojirimycin (NB-DNJ) is an inhibitor of human immunodeficiency virus (HIV) replication and HIV-induced syncytium formation in vitro. Although NB-DNJ appears to inhibit HIV entry at the level of post-CD4 binding (P.B. Fischer, M. Collin, G.B. Karlsson, W. James, T.D. Butters, S.J. Davis, S. Gordon, R.A. Dwek, and F.M. Platt, J. Virol. 69:5791-5797, 1995), the exact mechanism of action remains to be established. In this study we have examined the effect of NB-DNJ on the structure of recombinant gp120 (rgpl20), expressed in CHO cells, by using a panel of 40 monoclonal antibodies. The levels of binding of antibodies to rgp120 produced in the presence [rgpl20(+)] and absence [rgpl20(-)] of NB-DNJ were compared by enzyme-linked immunosorbent assay and surface plasmon resonance (BIAcore; Pharmacia). The results showed an increase in the binding to rgp120(+) of antibodies directed against the C1 and C2 regions and a decrease in the binding of antibodies directed against the V1/V2 loops compared with antibody binding to rgpl20(-). A decrease in the binding to rgpl20(+) of antibodies directed against discontinuous epitopes was also observed. No differences were seen in the binding of antibodies directed against the crown of the V3 loop and the C4 region of gp120. Treatment of rgpl20 with alpha-glucosidases I and II had no effect on the differential binding observed, whereas treatment with sialidase abolished the differences seen in the binding of antibodies directed against the C1 and C2 regions of gp120. In addition to these findings, rgpl20(+) showed increased sensitivity to proteases released by CHO cells during expression, as well as to exogenous thrombin. Taken together, the data presented in this paper suggest that production of gp120 in the presence of NB-DNJ affects the conformation of the Vl/V2 loops of gpl20, as well as the overall charge of the C1 and C2 regions. These effects may play a role in the previously described NB-DNJ-mediated inhibition of HIV entry at the level of post-CD4 binding.
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