ArticleJournal of virology1996
The vaccinia virus H5R gene encodes late gene transcription factor 4: purification, cloning, and overexpression.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 80 citations in OpenAlex.
- Poxvirus H5 mediates the formation of liquid-liquid phase separation condensates which promote virus factory assembly.PLoS pathogens · 2025Article
- Transcription termination and readthrough in African swine fever virus.Frontiers in immunology · 2024Article
- Deciphering poxvirus gene expression by RNA sequencing and ribosome profiling.Journal of virology · 2015Article
- Genetic Confirmation that the H5 Protein Is Required for Vaccinia Virus DNA Replication.Journal of virology · 2015Article
- Cascade regulation of vaccinia virus gene expression is modulated by multistage promoters.Virology · 2013Article
- Barrier to autointegration factor (BAF) inhibits vaccinia virus intermediate transcription in the absence of the viral B1 kinase.Virology · 2013Article
- Attenuated and replication-competent vaccinia virus strains M65 and M101 with distinct biology and immunogenicity as potential vaccine candidates against pathogens.Journal of virology · 2013Article
- Biochemical and biophysical properties of a putative hub protein expressed by vaccinia virus.The Journal of biological chemistry · 2013Article
- Vaccinia virus A6 is essential for virion membrane biogenesis and localization of virion membrane proteins to sites of virion assembly.Journal of virology · 2012Article
- RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cells.Virology journal · 2012Article
- Participation of vaccinia virus l2 protein in the formation of crescent membranes and immature virions.Journal of virology · 2011Article
- Capturing the natural diversity of the human antibody response against vaccinia virus.Journal of virology · 2011Article
- Article
- Vaccinia virus H7 protein contributes to the formation of crescent membrane precursors of immature virions.Journal of virology · 2009Article
- Vaccinia virus G8R protein: a structural ortholog of proliferating cell nuclear antigen (PCNA).PloS one · 2009Article
- Article
- Mapping and phenotypic analysis of spontaneous isatin-beta-thiosemicarbazone resistant mutants of vaccinia virus.Virology · 2007Article
- Vaccinia virus proteome: identification of proteins in vaccinia virus intracellular mature virion particles.Journal of virology · 2006Article
- Orthopoxvirus targets for the development of antiviral therapies.Current drug targets. Infectious disorders · 2005Review
- Vaccinia virus mutants with alanine substitutions in the conserved G5R gene fail to initiate morphogenesis at the nonpermissive temperature.Journal of virology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vaccinia virus late stage-specific transcription factor P3 was purified to homogeneity from HeLa cells that were infected in the presence of an inhibitor of viral DNA replication. The purified 36-kDa protein was digested with trypsin, and the peptides were analyzed by mass spectroscopy and amino-terminal sequencing. The purified factor was identified as the product of the vaccinia virus H5R open reading frame by both methods. A recombinant baculovirus was engineered to express the H5R open reading frame. The partially purified recombinant protein could replace the vaccinia virus P3 factor in transcription assays. On the basis of these findings, we assigned the H5R gene product the name viral late gene transcription factor 4 (VLTF-4). Unlike VLTF-1, -2, and -3, which are synthesized exclusively after viral DNA replication, VLTF-4 is synthesized before and after viral DNA synthesis. Indirect immunofluorescence of infected cells with anti-H5R protein antiserum demonstrated that VLTF-4 is diffusely distributed in the cytoplasm when DNA replication is blocked but is localized to discrete viral DNA-containing factories during a productive infection. Its expression pattern and subcellular distribution suggest that the H5R gene product may have multiple roles in the viral life cycle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.