ArticleJournal of virology1996
Recombinant adeno-associated virus-mediated high-efficiency, transient expression of the murine cationic amino acid transporter (ecotropic retroviral receptor) permits stable transduction of human HeLa cells by ecotropic retroviral vectors.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Long-term engraftment and angiogenic properties of lentivirally transduced adipose tissue-derived stromal cells.Molecular biotechnology · 2013Article
- Transduction of human primitive repopulating hematopoietic cells with lentiviral vectors pseudotyped with various envelope proteins.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Transduction of human embryonic stem cells by ecotropic retroviral vectors.Nucleic acids research · 2006Article
- Novel adeno-associated virus vector vaccine restricts replication of simian immunodeficiency virus in macaques.Journal of virology · 2005Article
- Genetic fate of recombinant adeno-associated virus vector genomes in muscle.Journal of virology · 2003Article
- Adaptation of chimeric retroviruses in vitro and in vivo: isolation of avian retroviral vectors with extended host range.Journal of virology · 2001Article
- Selective transduction of HIV-1-infected cells by the combination of HIV and MMLV vectors.International journal of hematology · 2001Article
- Design and packaging of adeno-associated virus gene targeting vectors.Journal of virology · 2000Article
- Article
- High-fidelity correction of mutations at multiple chromosomal positions by adeno-associated virus vectors.Journal of virology · 1999Article
- Adeno-associated virus vectors and hematology.Blood · 1999Review
- Adenovirus-mediated expresssion of the murine ecotropic receptor facilitates transduction of human hematopoietic cells with an ecotropic retroviral vector.Gene therapy · 1999Article
- Reduction in levels of the cyclin-dependent kinase inhibitor p27(kip-1) coupled with transforming growth factor beta neutralization induces cell-cycle entry and increases retroviral transduction of primitive human hematopoietic cells.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Human gene targeting by viral vectors.Nature genetics · 1998Article
- Infectious clones and vectors derived from adeno-associated virus (AAV) serotypes other than AAV type 2.Journal of virology · 1998Article
- Adeno-associated virus vector integration junctions.Journal of virology · 1997Article
- Development of high-titer retroviral producer cell lines by using Cre-mediated recombination.Journal of virology · 1997Article
- Adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cDNA allows ecotropic retroviral transduction of established and primary human cells.Journal of virology · 1997Article
- Recombinant adeno-associated virus mediates a high level of gene transfer but less efficient integration in the K562 human hematopoietic cell line.Journal of virology · 1997Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Adeno-associated virus has a broad host range, is nonpathogenic, and integrates into a preferred location on chromosome 19, features that have fostered development of recombinant adeno-associated viruses (rAAV) as gene transfer vectors for therapeutic applications. We have used an rAAV to transfer and express the murine cationic amino acid transporter which functions as the ecotropic retroviral receptor, thereby rendering human cells conditionally susceptible to infection by an ecotropic retroviral vector. The proportion of human HeLa cells expressing the receptor at 60 h varied as a function of the multiplicity of infection (MOI) with the rAAV. Cells expressing the ecotropic receptor were efficiently transduced with an ecotropic retroviral vector encoding a nucleus-localized form of beta-galactosidase. Cells coexpressing the ecotropic receptor and nucleus-localized beta-galactosidase were isolated by fluorescence-activated cell sorting, and cell lines were recovered by cloning at limiting dilution. After growth in culture, all clones contained the retroviral vector genome, but fewer than 10% (3 of 47) contained the rAAV genome and continued to express the ecotropic receptor. The ecotropic receptor coding sequences in the rAAV genome were under the control of a tetracycline-modulated promoter. In the presence of tetracycline, receptor expression was low and the proportion of cells transduced by the ecotropic retroviral vector was decreased. Modulation of receptor expression was achieved with both an episomal and an integrated form of the rAAV genome. These data establish that functional gene expression from an rAAV genome can occur transiently without genome integration.
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