ArticleProceedings of the National Academy of Sciences of the United States of America1996
Embryonic mesoderm cells spread in response to platelet-derived growth factor and signaling by phosphatidylinositol 3-kinase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Modeling the roles of cohesotaxis, cell-intercalation, and tissue geometry in collective cell migration of Xenopus mesendoderm.Biology open · 2024Article
- Article
- The myocardium utilizes Pdgfra-PI3K signaling to steer towards the midline during heart tube formation.bioRxiv : the preprint server for biology · 2023Article
- A Molecular Analysis of Cytokine Content across Extracellular Vesicles, Secretions, and Intracellular Space from Different Site-Specific Adipose-Derived Stem Cells.International journal of molecular sciences · 2021Article
- Imaging of dynamic actin remodeling reveals distinct behaviors of head and trunk mesoderm in gastrulatingmicroPublication biology · 2021Article
- Analysis of mice lacking the heparin-binding splice isoform of platelet-derived growth factor A.Molecular and cellular biology · 2013Article
- Protein phosphatase 1 β paralogs encode the zebrafish myosin phosphatase catalytic subunit.PloS one · 2013Article
- The cytoplasmic tyrosine kinase Arg regulates gastrulation via control of actin organization.Developmental biology · 2012Article
- Pair-wise regulation of convergence and extension cell movements by four phosphatases via RhoA.PloS one · 2012Article
- Disruption of platelet-derived growth factor-dependent phosphatidylinositol 3-kinase and phospholipase Cγ 1 activity abolishes vascular smooth muscle cell proliferation and migration and attenuates neointima formation in vivo.Journal of the American College of Cardiology · 2011Article
- The interferon-gamma-induced murine guanylate-binding protein-2 inhibits rac activation during cell spreading on fibronectin and after platelet-derived growth factor treatment: role for phosphatidylinositol 3-kinase.Molecular biology of the cell · 2010Article
- Reciprocal regulation of the platelet-derived growth factor receptor-beta and G protein-coupled receptor kinase 5 by cross-phosphorylation: effects on catalysis.Molecular pharmacology · 2009Article
- Role of platelet-derived growth factors in physiology and medicine.Genes & development · 2008Review
- Regulation of Xenopus gastrulation by ErbB signaling.Developmental biology · 2007Article
- siRNA-mediated simultaneous downregulation of uPA and its receptor inhibits angiogenesis and invasiveness triggering apoptosis in breast cancer cells.International journal of oncology · 2006Article
- Migrating anterior mesoderm cells and intercalating trunk mesoderm cells have distinct responses to Rho and Rac during Xenopus gastrulation.Developmental dynamics : an official publication of the American Association of Anatomists · 2006Article
- Regulation of early Xenopus development by ErbB signaling.Developmental dynamics : an official publication of the American Association of Anatomists · 2006Article
- alphaPIX nucleotide exchange factor is activated by interaction with phosphatidylinositol 3-kinase.Oncogene · 1999Article
- PI3K and Erk MAPK mediate ErbB signaling in Xenopus gastrulation.Mechanisms of developmentArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Abnormal mesoderm movement, leading to defects in axial organization, is observed in mouse and Xenopus laevis embryos deprived of platelet-derived growth factor (PDGF) AA signaling. However, neither the cellular response to PDGF nor the signaling pathways involved are understood. Herein we describe an in vitro assay to examine the direct effect of PDGF AA on aggregates of Xenopus embryonic mesoderm cells. We find that PDGF AA stimulates aggregates to spread on fibronectin. This behavior is similar to that of migrating mesoderm cells in vivo that spread and form lamellipodia and filipodia on contact with fibronectin-rich extracellular matrix. We go on to show two lines of evidence that implicate phosphatidylinositol 3-kinase (PI3K) as an important component of PDGF-induced mesoderm cell spreading. (i) The fungal metabolite wortmannin, which inhibits signaling by PI3K, blocks mesoderm spreading in response to PDGF AA. (ii) Activation of a series of receptors with specific tyrosine-to-phenylalanine mutations revealed PDGF-induced spreading of mesoderm cells depends on PI3K but not on other signaling molecules that interact with PDGF receptors including phospholipase C gamma, Ras GTPase-activating protein, and phosphotyrosine phosphatase SHPTP2. These results indicate that a PDGF signal, medicated by PI3K, can facilitate embryonic mesoderm cell spreading on fibronectin. We propose that PDGF, produced by the ectoderm, influences the adhesive properties of the adjacent mesoderm cells during gastrulation.
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