ArticleBiophysical journal1996
Fluorescence generalized polarization of cell membranes: a two-photon scanning microscopy approach.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Laurdan Adopts Distinct, Phase-Specific Orientations in Lipid Membranes.The journal of physical chemistry. B · 2025Article
- Abiotic lipid metabolism enables membrane plasticity in artificial cells.Nature chemistry · 2025Article
- Mapping membrane biophysical nano-environments.Nature communications · 2024Article
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.PLoS computational biology · 2024Article
- Membrane mimetic-dependence of GPCR energy landscapes.Structure (London, England : 1993) · 2024Article
- Gadolinium Effects on Liposome Fluidity and Size Depend on the Headgroup and Side Chain Structure of Key Mammalian Brain Lipids.Molecules (Basel, Switzerland) · 2023Article
- Membrane Mimetic-Dependence of GPCR Energy Landscapes.bioRxiv : the preprint server for biology · 2023Article
- Dual mechanisms of cholesterol-GPCR interactions that depend on membrane phospholipid composition.Structure (London, England : 1993) · 2023Article
- Quantification methods of determining brewer's and pharmaceutical yeast cell viability: accuracy and impact of nanoparticles.Analytical and bioanalytical chemistry · 2023Article
- Characterizing the Self-Assembly Properties of Monoolein Lipid Isosteres.The journal of physical chemistry. B · 2023Article
- Infrared Spectroscopic Study of Multi-Component Lipid Systems: A Closer Approximation to Biological Membrane Fluidity.Membranes · 2022Article
- Allosteric modulation of the adenosine AeLife · 2022Article
- Long-term STED imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes.Biophysical reports · 2021Article
- CD45 pre-exclusion from the tips of T cell microvilli prior to antigen recognition.Nature communications · 2021Article
- Fluorescence strategies for mapping cell membrane dynamics and structures.APL bioengineering · 2020Article
- Targeted fluorescence lifetime probes reveal responsive organelle viscosity and membrane fluidity.PloS one · 2019Article
- Dynamic cellular maps of molecular species: Application to drug-target interactions.Scientific reports · 2018Article
- An improved labeling strategy enables automated detection of single-virus fusion and assessment of HIV-1 protease activity in single virions.The Journal of biological chemistry · 2017Article
- New insight into the interaction of TRAF2 C-terminal domain with lipid raft microdomains.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Article
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4 authors.
Funding
Abstract
We use the lipophilic fluorescence probe Laurdan to study cell membranes. The generalized polarization (GP) of Laurdan-labeled cells contains useful information about membrane fluidity and polarity. A high GP is usually associated with low fluidity, low polarity, or high cholesterol content of the membranes, and a low GP is the opposite. We have combined the GP method and two-photon fluorescence microscopy to provide an alternative approach to study cell membranes. Using two-photon excitation in a conventional microscope offers great advantages for studying biological samples. These advantages include efficient background rejection, low photodamage, and improved depth discrimination. We performed GP measurements on mouse fibroblast cells and observed that both intensity and GP images are not spatially uniform. We tested for possible GP artifacts arising from cellular autofluorescence and lifetime quenching, using a procedure for background fluorescence subtraction and by direct lifetime measurements in the microscope. GP measured in a single cell displays a broad distribution, and the GP of 40 different cells grown on the same cover glass is also statistically distributed. The correlations between intensity and GP images were analyzed, and no monotonic dependence between the two was found. By digitally separating high and low GP values, we found that high GP values often associate with the regions of the plasma membrane and low GP values link with the nuclear membranes. Our results also show local GP variations within the plasma and nuclear membranes.
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