ArticleJournal of clinical microbiology1996
Molecular epidemiology of apparent outbreak of invasive aspergillosis in a hematology ward.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 108 citations in OpenAlex.
- Article
- Invasive Aspergillosis byJournal of fungi (Basel, Switzerland) · 2019Review
- Usefulness of Aspergillus Galactomannan Antigen Testing and the Prediction of an Outbreak during Hospital Reconstruction.Internal medicine (Tokyo, Japan) · 2018Article
- Genome Sequences of Three Strains ofGenome announcements · 2017Article
- Molecular characterization of Aspergillus infections in an Iranian educational hospital using RAPD-PCR method.Iranian journal of basic medical sciences · 2014Article
- Molecular epidemiology of Aspergillus fumigatus: an in-depth genotypic analysis of isolates involved in an outbreak of invasive aspergillosis.Journal of clinical microbiology · 2011Article
- Characterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America.Eukaryotic cell · 2007Article
- Comparison of two highly discriminatory molecular fingerprinting assays for analysis of multiple Aspergillus fumigatus isolates from patients with invasive aspergillosis.Journal of clinical microbiology · 2007Article
- Use of a novel panel of nine short tandem repeats for exact and high-resolution fingerprinting of Aspergillus fumigatus isolates.Journal of clinical microbiology · 2005Article
- Development of a method to detect and quantify Aspergillus fumigatus conidia by quantitative PCR for environmental air samples.Mycopathologia · 2004Article
- Identification to the species level and differentiation between strains of Aspergillus clinical isolates by automated repetitive-sequence-based PCR.Journal of clinical microbiology · 2004Article
- Epidemiology of Aspergillus terreus at a university hospital.Journal of clinical microbiology · 2003Article
- Evaluation of performance of four genotypic methods for studying the genetic epidemiology of Aspergillus fumigatus isolates.Journal of clinical microbiology · 2002Article
- Epidemiology and Prevention of Invasive Aspergillosis.Current infectious disease reports · 2001Article
- Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates.Journal of clinical microbiology · 2001Article
- Genetic polymorphism of Aspergillus fumigatus in clinical samples from patients with invasive aspergillosis: investigation using multiple typing methods.Journal of clinical microbiology · 2001Article
- Use of a repetitive DNA probe to type clinical and environmental isolates of Aspergillus flavus from a cluster of cutaneous infections in a neonatal intensive care unit.Journal of clinical microbiology · 2000Article
- Genetic similarity among one Aspergillus flavus strain isolated from a patient who underwent heart surgery and two environmental strains obtained from the operating room.Journal of clinical microbiology · 2000Article
- The ins and outs of DNA fingerprinting the infectious fungi.Clinical microbiology reviews · 2000Review
- Elucidating the origins of nosocomial infections with Candida albicans by DNA fingerprinting with the complex probe Ca3.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During a 2-month period, five patients suffering from invasive infections caused by Aspergillus flavus or Aspergillus fumigatus were identified in the Hematology Department of the University Hospital Dijkzigt (Rotterdam, The Netherlands). To study the epidemiological aspects of invasive aspergillosis, strains from these patients and from the hospital environment, isolated during extensive microbiological screening, were subjected to genotyping. A novel DNA extraction technique, involving freezing, grinding, and direct lysis in guanidium isothiocyanate-containing buffers of mycelial material, was applied. DNA isolation was followed by typing by random amplification of polymorphic DNA (RAPD) analysis. This showed that strains isolated from all patients infected with the same fungal species were genotypically distinct, thus providing evidence against the possibility of an ongoing, single-source nosocomial outbreak. Strains could also be differentiated from strains of geographically diverse origins. However, an A. flavus strain from one of the patients was also frequently encountered in the hospital environment. As all environmental strains were collected after this patient had been diagnosed with invasive disease, the epidemiological value of this observation could not be ascertained. Intensive investigations showed no single source of A. flavus or other aspergilli. RAPD genotyping proved that the outbreak of invasive aspergillosis in the hematology ward consisted of a series of unrelated events and was not due to a common source within the hospital. RAPD fingerprinting of aspergilli may greatly facilitate future investigations of the epidemiology of invasive disease caused by these pathogens.
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