ArticleJournal of clinical microbiology1996
Multiprimer PCR system for differential identification of mycobacteria in clinical samples.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression.BMC microbiology · 2005Pooled it
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- Zoonotic pathogens survey in free-living long-tailed macaques in Thailand.International journal of veterinary science and medicine · 2022Article
- Review
- Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis.Journal of microbiology (Seoul, Korea) · 2017Article
- Article
- Differentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments.BMC infectious diseases · 2016Article
- Article
- Development and evaluation of a molecular assay for detection of nontuberculous mycobacteria by use of the cobas amplicor platform.Journal of clinical microbiology · 2008Article
- Multiprimer PCR system diagnosis of pulmonary tuberculosis in Cochabamba, Bolivia.Journal of clinical microbiology · 2008Article
- Differentiation of Mycobacterial species by hsp65 duplex PCR followed by duplex-PCR-based restriction analysis and direct sequencing.Journal of clinical microbiology · 2006Article
- Microscopic-observation drug-susceptibility assay for the diagnosis of TB.The New England journal of medicine · 2006Article
- Use of the hupB gene encoding a histone-like protein of Mycobacterium tuberculosis as a target for detection and differentiation of M. tuberculosis and M. bovis.Journal of clinical microbiology · 2004Article
- Differential identification of Mycobacterium tuberculosis complex and nontuberculous mycobacteria by duplex PCR assay using the RNA polymerase gene (rpoB).Journal of clinical microbiology · 2004Article
- Identification of the causative organism of tuberculous lymphadenitis in ethiopia by PCR.Journal of clinical microbiology · 2002Article
- Assessment of mycobacterial, propionibacterial, and human herpesvirus 8 DNA in tissues of greek patients with sarcoidosis.Journal of clinical microbiology · 2002Article
- Specificities and functions of the recA and pps1 intein genes of Mycobacterium tuberculosis and application for diagnosis of tuberculosis.Journal of clinical microbiology · 2002Article
- Characterization of a Mycobacterium intracellulare variant strain by molecular techniques.Journal of clinical microbiology · 2001Article
- Duplex PCR for differential identification of Mycobacterium bovis, M. avium, and M. avium subsp. paratuberculosis in formalin- fixed paraffin-embedded tissues from cattle.Journal of clinical microbiology · 2000Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel multiprimer PCR method with the potential to identify mycobacteria in clinical samples is presented. The assay relies on the simultaneous amplification of three bacterial DNA genomic fragments by using different sets of oligonucleotide primers. The first set of primers amplifies a 506-bp fragment from the gene for the 32-kDa antigen of Mycobacterium tuberculosis, which is present in most of the species belonging to the genus Mycobacterium. The second set of primers amplifies a 984-bp fragment from the IS6110 insertion sequence of the bacteria belonging to the M. tuberculosis complex. The third set of primers, derived from an M. tuberculosis species-specific sequence named MTP40, amplifies a 396-bp genomic fragment. Thus, while the multiprimer system would render three amplification fragments from the M. tuberculosis genome and two fragments from the Mycobacterium bovis genome, a unique amplification fragment would be obtained from nontuberculous mycobacteria. The results obtained, using reference mycobacterial strains and typed clinical isolates, show that the multiprimer PCR method may be a rapid, sensitive, and specific tool for the differential identification of various mycobacterial strains in a single-step assay.
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