ArticleThe Journal of physiology1995
The actin filament disrupter cytochalasin D activates the recombinant cystic fibrosis transmembrane conductance regulator Cl- channel in mouse 3T3 fibroblasts.
Article in The Journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Lipid-driven CFTR clustering is impaired in cystic fibrosis and restored by corrector drugs.Journal of cell science · 2022Article
- Identification of Potential Leukocyte Biomarkers Related to Drug Recovery of CFTR: Clinical Applications in Cystic Fibrosis.International journal of molecular sciences · 2021Article
- The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Uses its C-Terminus to Regulate the A2B Adenosine Receptor.Scientific reports · 2016Article
- Article
- Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells.PloS one · 2015Article
- Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A.The Journal of biological chemistry · 2011Article
- Actin cytoskeleton-dependent dynamics of the human serotonin1A receptor correlates with receptor signaling.Biophysical journal · 2008Article
- CFTR surface expression and chloride currents are decreased by inhibitors of N-WASP and actin polymerization.Biochimica et biophysica acta · 2007Article
- Convergent regulation of skeletal muscle Ca2+ channels by dystrophin, the actin cytoskeleton, and cAMP-dependent protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Leptin activation of ATP-sensitive K+ (KATP) channels in rat CRI-G1 insulinoma cells involves disruption of the actin cytoskeleton.The Journal of physiology · 2000Article
- Efficient CFTR expression from AAV vectors packaged with promoters--the second generation.Gene therapy · 1999Article
- Lysophosphatidic acid stimulates neurotransmitter-like conductance changes that precede GABA and L-glutamate in early, presumptive cortical neuroblasts.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Efficient expression of CFTR function with adeno-associated virus vectors that carry shortened CFTR genes.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Cytochalasin D (CD; 5 microM) readily stimulated cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel activity in cell-attached and whole-cell patch recordings from 3T3 fibroblasts expressing recombinant CFTR but not in mock-transfected cells. CD-stimulated currents were indistinguishable from those evoked by forskolin stimulation. Kinetic analysis of CFTR gating showed identical channel behaviour independent of the agonist used. 2. To elucidate the mechanism of action of CD we tested its effects on cAMP, protein kinase A, and the CFTR itself during CD stimulation. In contrast to forskolin treatment, CD did not increase cellular cAMP content. 3. A direct interaction of CD with the CFTR was ruled out because CD showed no effect on CFTR in excised inside-out patches. 4. CD effects were fully blocked when the cellular protein kinase A was inhibited by treatment of cells with RpcAMPS in cell-attached patches or when protein kinase inhibitor peptide was dialysed into cells in whole-cell experiments. 5. Addition of G-actin to excised patches had no effects on CFTR. 6. We conclude that the stimulatory effect of CD is cAMP independent, but needs a functional protein kinase A in order to activate the CFTR. We propose that cytochalasin D activates CFTR by releasing a cellular inhibitor, e.g. a phosphatase, that is held in place by F-actin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.