ArticleThe Journal of clinical investigation1996
Cross-linking of the dermo-epidermal junction of skin regenerating from keratinocyte autografts. Anchoring fibrils are a target for tissue transglutaminase.
Article in The Journal of clinical investigation, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 102 citations in OpenAlex.
- Marine collagen for regenerative medicine: Structure-function advantages, limitations, and translational pathways.Bioactive materials · 2026Review
- The effect of macromolecular crowders as a supplement to serum free media on human corneal stromal cells proliferation and marker expression.Scientific reports · 2026Article
- Long-Term Outcomes of a Cultured Autologous Dermo-Epidermal Skin Substitute in Children: 5-Year Results of a Phase I Clinical Trial.Journal of burn care & research : official publication of the American Burn Association · 2025Article
- Mesenchymal Transglutaminase 2 Activates Epithelial ADAM17: Link to G-Protein-Coupled Receptor 56 (ADGRG1) Signalling.International journal of molecular sciences · 2024Article
- Dystrophic Epidermolysis Bullosa: Secondary Disease Mechanisms and Disease Modifiers.Frontiers in genetics · 2021Review
- Making more matrix: enhancing the deposition of dermal-epidermal junction components in vitro and accelerating organotypic skin culture development, using macromolecular crowding.Tissue engineering. Part A · 2015Article
- Loss of collagen VII is associated with reduced transglutaminase 2 abundance and activity.The Journal of investigative dermatology · 2014Article
- Tissue transglutaminase inhibits the TRPV5-dependent calcium transport in an N-glycosylation-dependent manner.Cellular and molecular life sciences : CMLS · 2012Article
- Epidermal transglutaminase (TGase 3) is required for proper hair development, but not the formation of the epidermal barrier.PloS one · 2012Article
- Transglutaminases (TGs) in ocular and periocular tissues: effect of muscarinic agents on TGs in scleral fibroblasts.PloS one · 2011Article
- Skingineering I: engineering porcine dermo-epidermal skin analogues for autologous transplantation in a large animal model.Pediatric surgery international · 2011Article
- Modular enzymatically crosslinked protein polymer hydrogels for in situ gelation.Biomaterials · 2010Article
- Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells.Pediatric surgery international · 2010Article
- Increased TG2 expression can result in induction of transforming growth factor beta1, causing increased synthesis and deposition of matrix proteins, which can be regulated by nitric oxide.The Journal of biological chemistry · 2009Article
- Supramolecular interactions in the dermo-epidermal junction zone: anchoring fibril-collagen VII tightly binds to banded collagen fibrils.The Journal of biological chemistry · 2008Article
- Enhanced expression of transglutaminase 2 in anterior polar cataracts and its induction by TGF-beta in vitro.The British journal of ophthalmology · 2002Article
- Facilitated wound healing by activation of the Transglutaminase 1 gene.The American journal of pathology · 2000Article
- Tissue-specific expression and long-term deposition of human collagen VII in the skin of transgenic mice: implications for gene therapy.Gene therapy · 2000Article
- Implication of tissue transglutaminase and desmoplakin in cell adhesion mechanism in human epidermis.Molecular and cellular biochemistry · 2000Article
- Cultured epithelial autografts: diving from surgery into matrix biology.Pediatric surgery international · 1997Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since transglutaminases create covalent gamma-glutamyl-epsilon-lysine cross-links between extracellular matrix proteins they are prime candidates for stabilizing tissue during wound healing. Therefore, we studied the temporo-spatial expression of transglutaminase activity in skin regenerating from cultured epithelial autografts in severely burned children by the specific incorporation of monodansylcadaverine into cryostat sections from skin biopsies obtained between 5 d to 17 mo after grafting. The dansyl label was subsequently immunolocalized in the epidermis, dermal connective tissue, and along the basement membrane. Incubation of cryosections of normal and regenerating skin with purified tissue transglutaminase confirmed the dermo-epidermal junction and the papillary dermis as targets for this enzyme and revealed that in regenerating skin transamidation of the basement membrane zone was completed only 4-5 mo after grafting. Immunoelectron microscopy revealed that three distinct regions on the central portion of anchoring fibrils were positive for monodansylcadaverine in normal skin which were negative during the initial phase of de novo formation of anchoring fibrils in regenerating skin. Biochemically, we identified collagen VII as potential substrate for tissue transglutaminase. Thus, tissue transglutaminase appears to play an important role not only in cross-linking of the papillary dermis but also of the dermo-epidermal junction in particular.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.