ArticleApplied and environmental microbiology1996
Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Base editor enables rational genome-scale functional screening for enhanced industrial phenotypes inScience advances · 2022Article
- A novel SfaNI-like restriction-modification system in Caldicellulosiruptor extents the genetic engineering toolbox for this genus.PloS one · 2022Article
- Occurrence of randomly recombined functional 16S rRNA genes in Thermus thermophilus suggests genetic interoperability and promiscuity of bacterial 16S rRNAs.Scientific reports · 2019Article
- Establishment of uracil auxotrophic dikaryotic strains ofBreeding science · 2017Article
- Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant.Applied and environmental microbiology · 2010Article
- Article
- Characterization of three putative Lon proteases of Thermus thermophilus HB27 and use of their defective mutants as hosts for production of heterologous proteins.Extremophiles : life under extreme conditions · 2008Article
- Orotate phosphoribosyltransferase from Corynebacterium ammoniagenes lacking a conserved lysine.Journal of bacteriology · 2007Article
- Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain.Nucleic acids research · 2001Article
- Thermostable repair enzyme for oxidative DNA damage from extremely thermophilic bacterium, Thermus thermophilus HB8.Nucleic acids research · 1998Article
- A new Thermus-Escherichia coli shuttle integration vector system.Journal of bacteriology · 1997Article
- Structure and expression of a pyrimidine gene cluster from the extreme thermophile Thermus strain ZO5.Journal of bacteriology · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated uracil auxotrophic mutants of an extreme thermophile, Thermus thermophilus. A part of the pyrimidine biosynthetic operon including genes for orotate phosphoribosyltransferase (pyrE) and for orotidine-5'-monophosphate decarboxylase (pyrF) was cloned and sequenced. The pyrE gene can be a bidirectional marker for the gene manipulation system of the thermophile.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.