ArticleApplied and environmental microbiology1996
Generation of polyclonal antibodies against nisin: immunization strategies and immunoassay development.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
- In Vivo Antimicrobial Activity of Nisin Z AgainstAntibiotics (Basel, Switzerland) · 2025Article
- Probiotics at War Against Viruses: What Is Missing From the Picture?Frontiers in microbiology · 2020Review
- Cloning and heterologous production of Hiracin JM79, a Sec-dependent bacteriocin produced by Enterococcus hirae DCH5, in lactic acid bacteria and Pichia pastoris.Applied and environmental microbiology · 2008Article
- A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example.Journal of Zhejiang University. Science. B · 2007Article
- Immunochemical characterization of temperature-regulated production of enterocin L50 (EntL50A and EntL50B), enterocin P, and enterocin Q by Enterococcus faecium L50.Applied and environmental microbiology · 2006Article
- Determination of bacteriocin activity with bioassays carried out on solid and liquid substrates: assessing the factor "indicator microorganism".Microbial cell factories · 2006Article
- Bioassay for nisin in milk, processed cheese, salad dressings, canned tomatoes, and liquid egg products.Applied and environmental microbiology · 2006Article
- Effects of Lactococcus lactis on composition of intestinal microbiota: role of nisin.Applied and environmental microbiology · 2006Article
- Immunodetection of the bacteriocin lacticin RM: analysis of the influence of temperature and Tween 80 on its expression and activity.Applied and environmental microbiology · 2004Article
- Microplate bioassay for nisin in foods, based on nisin-induced green fluorescent protein fluorescence.Applied and environmental microbiology · 2003Article
- Detection of the bacteriocin propionicin PLG-1 with polyvalent anti-PLG-1 antiserum.Applied and environmental microbiology · 2001Article
- Heterologous coproduction of enterocin A and pediocin PA-1 by Lactococcus lactis: detection by specific peptide-directed antibodies.Applied and environmental microbiology · 2000Article
- Enhanced production of pediocin PA-1 and coproduction of nisin and pediocin PA-1 by Lactococcus lactis.Applied and environmental microbiology · 1999Article
- A nisin bioassay based on bioluminescence.Applied and environmental microbiology · 1999Article
- Generation of polyclonal antibodies of predetermined specificity against pediocin PA-1.Applied and environmental microbiology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Murine polyclonal antibodies reactive to the lantibiotic bacteriocin nisin A (nisA) have been produced by immunization with nisA-cholera toxin and nisA-keyhole limpet hemocyanin (nisA-KLH) conjugates. Mice immunized with nisA-cholera toxin developed nisA-specific antibodies with low relative affinities and poor sensitivities, while the immunization of mice with nisA-KLH conjugates resulted in the production of nisA-specific antibodies with high relative affinities and much-increased sensitivities. nisA antibodies could also be readily mass produced in less than 8 weeks in ascites fluid by using the nisA-KLH conjugate. A competitive direct enzyme-linked immunosorbent assay (ELISA) whereby nisA-horseradish peroxidase and free nisA competed for antibody binding was devised. The detection limit for nisA in the competitive direct ELISA with the nisA-KLH-generated antibodies was from 5 to 100 ng/ml, while the amount of free nisA required for 50% antibody binding inhibition ranged from 0.3 to 5 micrograms /ml. Both antisera and ascites polyclonal antibodies cross-reacted with nisZ either in the supernatant of a producer strain or with the pure lantibiotic but did not cross-react at all with non-lantibiotic-type bacteriocins. These polyclonal antibodies should find a wide usage from nisA ELISA analysis in foods and other matrices.
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