ArticleApplied and environmental microbiology1996
Typing of rhizobia by PCR DNA fingerprinting and PCR-restriction fragment length polymorphism analysis of chromosomal and symbiotic gene regions: application to Rhizobium leguminosarum and its different biovars.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Host-specific fluorescence dynamics in legume-rhizobium symbiosis during nodulation.Applied and environmental microbiology · 2026Article
- Diverse Peanut Bradyrhizobial Communities in Chinese Soils: Insights from Eastern, Central, and Northern Henan Province.Microbial ecology · 2025Article
- Increased diversity of beneficial rhizobia enhances faba bean growth.Nature communications · 2024Article
- Phylogenetic diversity of Rhizobium species recovered from nodules of common beans (Phaseolus vulgaris L.) in fields in Uganda: R. phaseoli, R. etli, and R. hidalgonense.FEMS microbiology ecology · 2024Article
- Phenotypic and genotypic diversity of root nodule bacteria from wild Lathyrus and Vicia species in Gaziantep, Turkey.Folia microbiologica · 2024Article
- Unearthing Optimal Symbiotic Rhizobia Partners from the Main Production Area ofInternational journal of molecular sciences · 2024Article
- Geographical and climatic distribution of lentil-nodulating rhizobia in Iran.FEMS microbiology ecology · 2024Article
- DiversePlants (Basel, Switzerland) · 2023Article
- Genotypic composition and performance of pea-nodulating rhizobia from soils outside the native plant-host range.Frontiers in microbiology · 2023Article
- Towards inoculant development for Bambara groundnut (Frontiers in plant science · 2023Article
- Arachis hypogaea L. from Acid Soils of Nanyang (China) Is Frequently Associated with Bradyrhizobium guangdongense and Occasionally with Bradyrhizobium ottawaense or Three Bradyrhizobium Genospecies.Microbial ecology · 2022Article
- New Insights into the Taxonomy of Bacteria in the Genomic Era and a Case Study with Rhizobia.International journal of microbiology · 2022Review
- Diversification ofFrontiers in microbiology · 2022Article
- Diversity of rhizobial and non-rhizobial bacteria nodulating wild ancestors of grain legume crop plants.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Root Nodule Rhizobia From Undomesticated Shrubs of the Dry Woodlands of Southern Africa Can Nodulate Angolan TeakFrontiers in microbiology · 2021Article
- Significance of Plant Growth Promoting Rhizobacteria in Grain Legumes: Growth Promotion and Crop Production.Plants (Basel, Switzerland) · 2020Review
- Molecular Diversity Analysis of Plant Growth Promoting Rhizobium Isolated from Groundnut and Evaluation of Their Field Efficacy.Current microbiology · 2020Article
- Rhizobia Isolated from the Relict LegumeGenes · 2019Article
- Genomic polymorphism of Trifolium repens root nodule symbionts from heavy metal-abundant 100-year-old waste heap in southern Poland.Archives of microbiology · 2019Article
- Phylogenetic diversity and cross-inoculation of indigenous isolatedMolecular biology research communications · 2019Article
1 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
Characterization of 43 strains of Rhizobium leguminosarum biovars viciae, trifolii, and phaseoli was performed by two methodologies based on PCR amplification, i.e., PCR DNA fingerprinting of interrepeat sequences and restriction fragment length polymorphism (RFLP) analysis of PCR -amplified chromosomal and symbiotic gene regions. Groupings generated by PCR DNA fingerprinting with either extragenic palindromic repetitive primers or two different single random primers were correlated with similar levels of resolution. Although less discriminating, PCR-RFLP analysis of intergenic spacer between genes coding for 16S and 23S rRNA (16S and 23S rDNA) yielded intraspecific polymorphisms. The classification of strains was independent of the biovar status and was in agreement with those obtained by PCR DNA fingerprinting. Intrabiovar variation within symbiotic gene regions was detected by PCR-RFLP analysis of nifDK and nodD gene regions, but the strains were grouped according to the biovar. The rDNA intergenic spacer and nif primers were verified to be universal for rhizobial species by testing of various reference strains, whereas the nod primers designed in this study were biovar or species specific for R. leguminosarum and Rhizobium etli. Classifications of R. leguminosarum strains by the PCR-based methods were correlated with those previously obtained by conventional total DNA restriction profile comparisons and RFLP analysis using chromosomal and symbiotic gene probes. Ranges of discriminating powers were also equivalent between the two approaches. However, the PCR-based methods are much less time-consuming and are therefore more convenient.
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